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Genetic variation, morphology and pathogenicity of Ceratocystis fimbriata on Hevea brasiliensis in Brazil

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Abstract

Ceratocystis fimbriata causes diseases on a wide variety of plants in Brazil, including rubber tree (Hevea brasiliensis), on which it causes gray mold or moldy rot on tapping panels affecting latex yield. However, C. fimbriata isolated from rubber tree have not been critically studied. Phylogenetic analyses of sequences of ITS rDNA and a mating type gene placed rubber tree isolates from Acre and Bahia among Brazilian isolates of C. fimbriata from other hosts. In the analyses of 14 microsatellite loci, the rubber tree isolates from Bahia were identical to each other and had alleles similar to those of Brazilian isolates from mango and eucalyptus. The microsatellite alleles of the Acre rubber tree isolates were identical to each other but distinct from other Brazilian isolates. The rubber tree isolates were morphologically indistinguishable from each other and very similar to the isolates of C. fimbriata on Ipomoea batatas, on which the species was originally described. Based on inoculation experiments results, the Bahia and Acre rubber tree isolates do not appear to be host specialized, which is typical for Brazilian isolates of C. fimbriata sensu stricto.

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References

  • Albuquerque FC, Duarte MLR, Silva HM (1972) Ocorrência do mofo cinzento (Ceratocystis fimbriata) da seringueira. In: Seminário Nacional da Seringueira. Cuiabá, MS. p. 125–128

  • Alfenas AC, Ferreira FA, Mafia RG, Gonçalves RC (2007) Isolamento de fungos fitopatogênicos. In: Alfenas AC, Mafia RG. Métodos em Fitopatologia. Editora UFV, Viçosa

  • Baker CJ, Harrington TC, Krauss U, Alfenas AC (2003) Genetic variability and host specialization in the Latin American clade of Ceratocystis fimbriata. Phytopathology 93:1274–1284

    Article  CAS  PubMed  Google Scholar 

  • Carvalho MB (1938) Sobre dois insetos nocivos à mangueira. Bol Secr Agric Ind Comércio Estado Pernamb 3:130–132

    Google Scholar 

  • Engelbrecht CJ, Harrington TC (2005) Intersterility, morphology and taxonomy of Ceratocystis fimbriata on sweet potato, cacao and sycamore. Mycologia 97:57–69

    Article  PubMed  Google Scholar 

  • Engelbrecht CJ, Harrington TC, Steimel J, Capretti P (2004) Genetic variation in eastern North American and putatively introduced populations of Ceratocytsis fimbriata f. platani. Mol Ecol 13:2995–3005

    Article  CAS  PubMed  Google Scholar 

  • Engelbrecht CJ, Harrington TC, Alfenas AC, Suarez C (2007) Genetic variation in populations of the cacao wilt pathogen, Ceratocystis cacaofunesta. Plant Pathol 56:923–933

    Article  CAS  Google Scholar 

  • Ferreira FA, Maffia LA, Barreto RW, Demuner NL, Pigatto S (2006) Sintomatologia da murcha de Ceratocystis fimbriata em eucalipto. Rev Árvore 2:155–162

    Google Scholar 

  • Ferreira EM, Harrington TC, Thorpe DJ, Alfenas AC (2010) Genetic diversity and interfertility among highly differentiated populations of Ceratocystis fimbriata in Brazil. Plant Pathol 59:721–735

    Article  CAS  Google Scholar 

  • Ferreira MA, Harrington TC, Alfenas AC, Mizubuti ESG (2011) Movement of genotypes of Ceratocystis fimbriata within and among Eucalyptus plantations in Brazil. Phytopathology 101:1005–1012

    Article  PubMed  Google Scholar 

  • Ferreira MA, Harrington TC, Gongora-Canul CC, Mafia RG, Zauza EAV, Alfenas AC (2013) Spatial-temporal patterns of Ceratocystis wilt in Eucalyptus plantations in Brazil. For Pathol 43:153–164

    Article  Google Scholar 

  • Figueiredo P, Pinheiro ED (1969) Uma nova doença da figueira (Ficus carica L.) na região de Valinhos, SP. Biológico 35:227--203

    Google Scholar 

  • Furtado EL (2007) Doenças da seringueira e seu manejo no Brasil. Informe Agrop 237:78–87

    Google Scholar 

  • Halsted BD (1890) Some fungus diseases of the sweet potato. The black rot. N J Agric Exp Station Bull 76:7–14

    Google Scholar 

  • Halsted BD, Fairchild DG (1891) Sweet-potato black rot. J Mycol 7:1–11

    Article  Google Scholar 

  • Harrington TC, McNew DL (1997) Self-fertility and unidirectional mating type switching in Ceratocystis coerulescens, a filamentous ascomycete. Curr Genet 32:52–59

    Article  CAS  PubMed  Google Scholar 

  • Harrington TC, Thorpe DJ, Alfenas AC (2011) Genetic variation and variation in aggressiveness to native and exotic hosts among Brazilian populations of Ceratocystis fimbriata. Phytopathology 101:555–566

    Article  PubMed  Google Scholar 

  • Harrington TC, Kazmi MR, Al-Sadi AM, Ismail SI (2014) Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocytsis fimbriata sensu stricto. Mycologia 106:224–242

    Article  CAS  PubMed  Google Scholar 

  • Harrington TC, Huang Q, Ferreira MA, Alfenas AC (2015) Genetic analyses trace the Yunnan, China population of Ceratocystis fimbriata on pomegranate and taro to populations on Eucalyptus in Brazil. Plant Dis 99:106–111

    Article  Google Scholar 

  • Laia ML, Alfenas AC, Harrington TC (2000) Isolation, detection in soil, and inoculation of Ceratocystis fimbriata, causal agent of wilting, die-back and canker in Eucalyptus. Fitopatol Bras 25:384

    Google Scholar 

  • McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential and durable resistance. Annu Rev Phytopathol 40:349–379

    Article  CAS  PubMed  Google Scholar 

  • Muchovej JJ, Albuquerque FC, Ribeiro GT (1978) Gmelina arborea – a new host of Ceratocystis fimbriata. Plant Dis Report 62:717–719

    Google Scholar 

  • Nkuekam GK, Barnes I, Wingfield MJ, Roux J (2009) Distribution and population diversity of Ceratocystis pirilliformis in South Africa. Mycologia 101:17–25

    Article  CAS  Google Scholar 

  • Nylander JAA (2004) MrModeltest Version 2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala

    Google Scholar 

  • Ocasio-Morales RG, Tsopelas P, Harrington TC (2007) Origin of Ceratocystis platani on native Platanus orientalis in Greece and its impact on natural forests. Plant Dis 91:901–904

    Article  Google Scholar 

  • Pereira JCR, Santos AF (1985) Controle químico do mofo cinzento no painel de sangria da seringueira. Comunicado técnico. n.46. Manuas, AM. Embrapa.

  • Pyenson L (1938) Problems of applied entomology in Pernambuco. II A survey of some of the pests of the crops of Pernambuco. Rev Entomol 9:16–31

    Google Scholar 

  • Rambaut A, Drummond AJ (2007) Tracer v1.4.. Available at: http://beast.bio.ed.ac.uk/Tracer. Accessed date: 15 October, 2014.

  • Ribeiro GT (1982) Avaliação preliminar da resistência de árvores de Gmelina arborea Lineaus, mediante inoculações do fungo Ceratocystis fimbriata El. & Halst., causador do cancro em gmelina. Fitopatol Bras 7:517

    Google Scholar 

  • Ribeiro IJA, Ito MF, Rossetto CJ (1984) Cassia renigera Wall., um novo hospedeiro de Ceratocystis fimbriata Ell. & Halst. Fitopatol Bras 9:322

    Google Scholar 

  • Ribeiro IJA, Ito MF, Rossetto CJ (1987) Cassia renigera Wall.: novo hospedeiro de Ceratocystis fimbriata Ell. & Halst. Bragantia 46:417–423

    Article  Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  CAS  PubMed  Google Scholar 

  • Rossetto CJ, Ribeiro IJA (1990) Mango wilt. XII. Recommendations for control. Rev Agric 65:173–180

    Google Scholar 

  • Silveira AP, Cardoso RMG, Neto FB, Oliveira DA (1985) Ocorrência e controle químico do mofo cinzento (Ceratocystis fimbriata Ell. & Hast.) da seringueira. Fitopatol Bras 10:281

    Google Scholar 

  • Steimel J, Engelbrecht CJB, Harrington TC (2004) Development and characterization of microsatellite markers for fungus Ceratocystis fimbriata. Mol Ecol Notes 4:215–218

    Article  CAS  Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson JD, Higgins D, Gibson TJ (1992) CLUSTAL versionW: a novel multiple sequence alignment program. Nucleic Acids Res 22:4673–4680

    Article  Google Scholar 

  • van Wyk M, van der Merve NA, Roux I, Wingfield BD, Kamgan GN, Wingfield MJ (2006) Population genetic anlyses suggest that the Eucalyptus fungal pathogen Ceratocystis fimbriata has been introduced into South Africa. S Afr J Sci 102:259–263

    Google Scholar 

  • van Wyk M, Al Adawi AO, Khan IA, Deadman ML, Al Jahwari AA, Wingfield BD, Ploetz R, Wingfield MJ (2007) Ceratocystis manginecans sp. nov., causal agent of a destructive mango wilt disease in Oman and Pakistan. Fungal Divers 27:213–230

    Google Scholar 

  • van Wyk M, Wingfield BD, Mohali S, Wingfield MJ (2009) Ceratocystis fimbriatomima, a new species in the C. fimbriata sensu lato complex isolated from Eucalyptus trees in Venezuela. Fungal Divers 34:175–185

    Google Scholar 

  • van Wyk M, Wingfield BD, Marin M, Wingfield MJ (2010) New Ceratocystis species infecting coffee, cacao, citrus and native trees in Colombia. Fungal Divers 40:103–117

    Article  Google Scholar 

  • van Wyk M, Wingfield BD, Al-Adawi AO, Rossetto CJ, Ito MF, Wingfield MJ (2011a) Two new Ceratocystis species associated with mango disease in Brazil. Mycotaxon 117:381–404

    Article  Google Scholar 

  • van Wyk M, Wingfield BD, Wingfield MJ (2011b) Four new Ceratocystis spp. associated with wounds on Eucalyptus, Schizolobium and Terminalia trees in Ecuador. Fungal Divers 46:111–163

    Article  Google Scholar 

  • van Wyk M, Roux J, Nkuekam GK, Wingfield BD, Wingfield MJ (2012) Ceratocystis eucalypticola sp. nov. from Eucalyptus in South Africa and comparison to global isolates from this tree. IMA Fungus 3:45–58

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This work was supported by CNPq, FAPEMIG and CAPES. We would like to thank Dr. Rivadalve Goncalves (Embrapa Acre) and Dr. Carlos Mattos (Michelin) for technical assistance during the collection of isolates. Also, we are thankful to Dr. Dalmo L. Siqueira and the companies Michelin and Tecnoplanta for providing plant material for the inoculations and Clonar Resistência a Doenças Florestais for the plant growth and inoculation facilities.

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Authors and Affiliations

  1. Dep. de Fitopatologia, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Denise C. O. F. Valdetaro, Leonardo S. S. Oliveira, Lúcio M. S. Guimarães, Rodrigo G. Freitas & Acelino C. Alfenas

  2. Department of Plant Pathology and Microbiology, Iowa State University, Ames, 50011, IA, USA

    Thomas C. Harrington

  3. Dep. de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, 37200-000, Brazil

    Maria A. Ferreira

Authors
  1. Denise C. O. F. Valdetaro
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  2. Leonardo S. S. Oliveira
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  3. Lúcio M. S. Guimarães
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  4. Thomas C. Harrington
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  5. Maria A. Ferreira
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  6. Rodrigo G. Freitas
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  7. Acelino C. Alfenas
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Correspondence to Acelino C. Alfenas.

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Section Editor: Silvaldo Silveira

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Valdetaro, D.C.O.F., Oliveira, L.S.S., Guimarães, L.M.S. et al. Genetic variation, morphology and pathogenicity of Ceratocystis fimbriata on Hevea brasiliensis in Brazil. Trop. plant pathol. 40, 184–192 (2015). https://doi.org/10.1007/s40858-015-0036-6

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  • DOI: https://doi.org/10.1007/s40858-015-0036-6

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