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The expression of microRNA-34a is inversely correlated with c-MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients

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Tumor Biology

Abstract

We aimed to establish whether the expression of microRNA-34a (miR-34a) is correlated with that of c-MET and G1 phase regulators such as cyclin dependent kinase (CDK) 4, CDK6, and cyclin D (CCND) 1 in non-small cell lung cancer (NSCLC), and whether a relationship exists between miR-34a expression and both clinicopathologic factors and recurrence-free survival (RFS). For 58 samples archived from NSCLC patients, we measured the expression of miR-34a and c-MET, CDK4/6, and CCND1 by quantitative RT-PCR and assessed the relationship between miR-34a expression, clinicopathological factors, and RFS. The expression of miR-34a was significantly lower in squamous cell tumors (P < 0.001) and in tumors associated with lymphatic invasion (P = 0.001). We found significant inverse correlations between miR-34a and c-MET (R = −0.316, P = 0.028) and CDK6 expression (R = −0.4582, P = 0.004). RFS were longer in adenocarcinoma patients with high miR-34a expression than in those with low miR-34a expression (55.6 vs. 21.6 months; P = 0.020). With univariate analysis, statistically significant prognostic factors for RFS in adenocarcinoma patients were miR-34a expression (Relative risk (RR), 8.14; P = 0.049), TNM stage (RR, 13.55; P = 0.001), LN metastasis (RR, 4.19; P = 0.043), and the presence of lymphatic invasion (RR, 7.05; P = 0.015). In multivariate analysis, only miR-34a was prognostic for RFS (RR, 11.5; P = 0.027). miR-34a expression was inversely correlated with that of c-MET and CDK6 in NSCLC, and had prognostic significance for RFS, especially in adenocarcinoma patients.

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Authors and Affiliations

  1. Department of Internal Medicine, College of Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Banpo-Daero 222, Seocho-Gu, Seoul, 137-701, Republic of Korea

    Ji Hyung Hong, Jae Ho Byun & Jin Hyoung Kang

  2. Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea

    Kang San Roh & Sukchan Lee

  3. South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje, Republic of Korea

    Sung-Suk Suh

  4. Department of Thoracic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea

    Sook Whan Sung & Jae Kil Park

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  1. Ji Hyung Hong
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Correspondence to Jin Hyoung Kang.

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Hong, J.H., Roh, K.S., Suh, SS. et al. The expression of microRNA-34a is inversely correlated with c-MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients. Tumor Biol. 36, 9327–9337 (2015). https://doi.org/10.1007/s13277-015-3428-9

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