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Differentially expressed genes between intestinal- and diffuse-type gastric cancers

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Abstract

Backgrounds

The two main types of gastric cancer, intestinal and diffuse, have different etiologies and pathogeneses, indicating different mechanisms of histogenesis and carcinogenesis that remain poorly understood.

Methods

The expression and correlation patterns of 13 development-, differentiation-, proliferation-, and invasion- related genes were compared between 23 diffuseand 27 intestinal-type gastric cancers by quantitative RT-PCR. In addition, the most representative results were confirmed by western blot analysis and methylation analysis was also performed in the two gastric cancer types using methylation-specific PCR.

Results

Seven genes, including SOX2, Muc5ac, CDH1, Muc4, TFF1, FGF7, and MMP10, with differential expression and correlation patterns between the two histological gastric cancer types were identified. Furthermore, Muc5ac, TFF1, MMP10, CDH1, and CDX2 expression levels were linked to DNA methylation status.

Conclusion

These data provide insights into type-specific gastric carcinogenesis, suggesting new approaches for a more profound investigation of the pathologic events that drive intestinal- and diffuse-type gastric cancer.

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Authors and Affiliations

  1. Department of Pathology, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea

    Olga Kim, Jung Hwan Yoon, Suk Woo Nam, Jung Young Lee & Won Sang Park

  2. Department of Medicine, Howard University, Washington, District of Columbia, 20060, USA

    Hassan Ashktorab

  3. Department of Medicine, Meharry Medical Center, Nashville, TN, 37208, USA

    Duane T. Smoot

Authors
  1. Olga Kim
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  2. Jung Hwan Yoon
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  3. Hassan Ashktorab
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  4. Duane T. Smoot
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  6. Jung Young Lee
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  7. Won Sang Park
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Correspondence to Won Sang Park.

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Kim, O., Yoon, J.H., Ashktorab, H. et al. Differentially expressed genes between intestinal- and diffuse-type gastric cancers. Mol. Cell. Toxicol. 14, 303–313 (2018). https://doi.org/10.1007/s13273-018-0033-1

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  • DOI: https://doi.org/10.1007/s13273-018-0033-1

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