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Evaluation of Cartilage Regeneration in Gellan Gum/agar Blended Hydrogel with Improved Injectability

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Abstract

Gellan gum is known to be one of the biocompatible materials that resemble living tissues, continuously delivering stem cells or general cells, and filling voids in the wound. In spite of the many advantages of gellan gum, it has difficulty in the application by inserting the scaffolds into the body. In order to generalize gellan gum for medical purpose, it is necessary to increase the injectability of gellan gum. In this study, gellan gum solutions with 0.2, 0.4, 0.6, 0.8 wt% of agar were prepared. The obtained hydrogels were tested for rheological properties and chondrocytes adhesion, morphology and proliferation. Results showed that the addition of agar, in the starting GG solution, allows extruding the material producing micro-porous scaffolds. The biological evaluation demonstrated that the adhesion and proliferation of chondrocytes increase with the presence of agar in the final structure. The solutions composed of GG and agar represent an efficient, fast and high reproducible methodology with the potential to be used as an injectable hydrogel for cartilage regeneration purposes.

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Authors and Affiliations

  1. Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Chonbuk National University, Deokjin-gu, Jeonju, 54896, Korea

    Jong Seon Baek, Thangavelu Muthukumar, David Kim, Jong Ho Park, Jeong Eun Song & Gilson Khang

  2. Department of Industrial Engineering, University of Trento, via Sommarive 9, Trento, Italy

    Cristiano Carlomagno, Claudio Migliaresi & Antonella Motta

  3. BIOTech Research Center, University of Trento, via delle Regole 101, Trento, Italy

    Cristiano Carlomagno, Claudio Migliaresi & Antonella Motta

  4. European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy

    Cristiano Carlomagno, Claudio Migliaresi & Antonella Motta

  5. B’s Research Group - Biomaterials, Biodegradable and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-17, Barco, Guimarães, Portugal

    Rui L. Reis

Authors
  1. Jong Seon Baek
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  2. Cristiano Carlomagno
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  3. Thangavelu Muthukumar
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  4. David Kim
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  5. Jong Ho Park
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  6. Jeong Eun Song
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  7. Claudio Migliaresi
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  8. Antonella Motta
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  9. Rui L. Reis
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  10. Gilson Khang
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Corresponding author

Correspondence to Gilson Khang.

Additional information

Acknowledgments: This research was supported by the International Research & Development Program through the National Research Foundation of Korea (NRF-2017K1A3A7A03089427) and a grant of the Korea Health Technology R&D Project (HI15C2996) through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea.

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Baek, J.S., Carlomagno, C., Muthukumar, T. et al. Evaluation of Cartilage Regeneration in Gellan Gum/agar Blended Hydrogel with Improved Injectability. Macromol. Res. 27, 558–564 (2019). https://doi.org/10.1007/s13233-019-7085-5

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  • DOI: https://doi.org/10.1007/s13233-019-7085-5

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