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Development of 31 polymorphic microsatellite markers for the mole salamander (Ambystoma talpoideum) using Illumina paired-end sequencing

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Abstract

We isolated and characterized a total of 31 microsatellite loci from the mole salamander, Ambystoma talpoideum. Loci were screened in 20 individuals from a single location in Aiken, South Carolina. The number of alleles per locus ranged from 3 to 11, observed heterozygosity ranged from 0.000 to 0.700, and the probability of identity values ranged from 0.031 to 0.400. These new loci will provide tools for examining the genetic diversity, structure, mating system, and adult morph determination of A. talpoideum.

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Acknowledgments

Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. We thank David Scott for assistance with sample collection.

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Authors and Affiliations

  1. Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC, 29802, USA

    Cara N. Love, R. Wesley Flynn, Schyler O. Nunziata & Stacey L. Lance

  2. Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, 80045, USA

    Kenneth L. Jones

Authors
  1. Cara N. Love
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  2. R. Wesley Flynn
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  3. Schyler O. Nunziata
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  4. Kenneth L. Jones
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  5. Stacey L. Lance
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Correspondence to Stacey L. Lance.

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Love, C.N., Flynn, R.W., Nunziata, S.O. et al. Development of 31 polymorphic microsatellite markers for the mole salamander (Ambystoma talpoideum) using Illumina paired-end sequencing. Conservation Genet Resour 5, 951–954 (2013). https://doi.org/10.1007/s12686-013-9940-6

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  • DOI: https://doi.org/10.1007/s12686-013-9940-6

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