Skip to main content
SpringerLink
Log in

Development of 15 microsatellite loci for Protoreaster nodosus (Echinodermata; Asteroidea)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Fifteen polymorphic microsatellite loci were developed for the horned sea star, Protoreaster nodosus, using 454 sequencing of microsatellite enriched genomic libraries. The 15 loci were characterized in 24 individuals from the Indonesian archipelago. We found a mean of 7 alleles per locus and observed levels of heterozygosity ranged from 0.042 to 0.875. All loci confirmed to Hardy–Weinberg expectations and no pairs of loci showed significant evidence of linkage disequilibrium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bos A, Gumanao G, Alipoyo J, Cardona L (2008) Population dynamics, reproduction and growth of the Indo-Pacific horned sea star, Protoreaster nodosus (Echinodermata; Asteroidea). Mar Biol 156:55–63

    Article  Google Scholar 

  • Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. Biotechniques 31:24–28

    PubMed  CAS  Google Scholar 

  • Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A (2010) Geneious v5.5, Available from http://www.geneious.com. Accessed 3 April 2012

  • Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res 10:564–567

    Article  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Method Enzym 395:202–222

    CAS  Google Scholar 

  • Hughes TP, Baird AH, Bellwood DR et al (2003) Climate change, human impacts, and the resilience of coral reefs. Science 301:929–933

    Article  PubMed  CAS  Google Scholar 

  • Jones GP, Srinivasan M, Almany GR (2007) Population connectivity and conservation of marine biodiversity. Oceanography 20:100–111

    Article  Google Scholar 

  • Meglecz E, Costedoat C, Dubut V et al (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404

    Article  PubMed  CAS  Google Scholar 

  • Scheibling RE, Metaxas A (2008) Abundance, spatial distribution, and size structure of the sea star Protoreaster nodosus in Palau, with notes on feeding and reproduction. Bull Mar Sci 82:221–235

    Google Scholar 

  • Toonen RJ (1997) Microsatellites for ecologists: non-radioactive isolation and amplification protocols for microsatellite markers. http://www2.hawaii.edu/~toonen/files/MsatsV1.pdf. Accessed 3 April 2012

  • Walsh PS, Metzger DA, Higuchi R (1991) Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513

    PubMed  CAS  Google Scholar 

  • Yamaguchi M (1973) Early life histories of coral reef asteroids, with special reference to Acanthaster planci (L.). In: Jones OA, Endean R (eds) Biology and geology of coral reefs. Academic Press, New York, pp 369–387

    Google Scholar 

Download references

Acknowledgments

We thank the Indonesian government, the Indonesian Institute of Sciences (LIPI) and RISTEK for collection permits, 0085/SIP/FRP/SM/V/2010 and 0133/SIP/FRP/SM/V/2010 under which all collections were made in full accordance with Indonesian laws and regulations. A special thank you is reserved for Mr Onny Nurrahman Marwayana and Ms Hagi Yulia Sugeha. Funding for this work was provided in part by the TOTAL Foundation, University of Hawaii Arts and Sciences Student Research Award, University of Hawaii Edmondson Grant, Sigma XI Grants In Aid of Research, the Explorers Club, the Rufford Small Grants Foundation and the University of Hawaii NSF-EPSCoR program. This is HIMB contribution # 1495 and the School for Ocean and Earth Science and Technology contribution # 8673.

Author information

Authors and Affiliations

  1. Hawai‘i Institute of Marine Biology, University of Hawai‘i, Mānoa, P.O. Box 1346, Mānoa, Kāne‘ohe, HI, 96744, USA

    Benjamin J. Wainwright & Stephen A. Karl

  2. Research Center for Oceanography, Indonesian Institute of Sciences, Jl. Pasir Putih I Ancol Timur, Jakarta, 14430, Indonesia

    Irma S. Arlyza

Authors
  1. Benjamin J. Wainwright
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Irma S. Arlyza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stephen A. Karl
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stephen A. Karl.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wainwright, B.J., Arlyza, I.S. & Karl, S.A. Development of 15 microsatellite loci for Protoreaster nodosus (Echinodermata; Asteroidea). Conservation Genet Resour 4, 873–875 (2012). https://doi.org/10.1007/s12686-012-9662-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-012-9662-1

Keywords

Search

Navigation