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Characterization of 10 polymorphic loci in the Baird’s pocket gopher (Geomys breviceps) and cross-amplification in other gopher species

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Abstract

Ten polymorphic microsatellite loci were isolated and characterized for the Baird’s pocket gopher, Geomys breviceps. Each locus was screened in 40 individuals from 4 different populations found throughout College Station, Texas. The number of alleles per locus ranged from 2 to 16 and observed heterozygosity ranged from 0.100 to 1.000. Deviation from Hardy–Weinberg and linkage disequilibrium was also analyzed. These 10 microsatellite primers, as well as 4 previously described primers developed for Thomomys, also were tested for amplification in additional species of pocket gophers. These microsatellite loci will provide useful tools in population genetic studies of G. breviceps, as well as other pocket gopher species.

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Acknowledgments

We would like to thank J. Gold, D. Portnoy, and J. Andersen for their assistance throughout the project. All animals collected within this study were treated humanely according to the guidelines provided by the American Society of Mammalogists (Gannon et al. 2007) and the Texas A&M Animal Care and Use Committee (IACUC). This is publication number 1423 of the Texas Cooperative Wildlife Collection and number 200 of the Center for Biosystematics and Biodiversity, both at Texas A&M University.

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Authors and Affiliations

  1. Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX, 77843, USA

    Sarah R. Welborn, Mark A. Renshaw & Jessica E. Light

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  1. Sarah R. Welborn
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  2. Mark A. Renshaw
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  3. Jessica E. Light
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Correspondence to Jessica E. Light.

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Welborn, S.R., Renshaw, M.A. & Light, J.E. Characterization of 10 polymorphic loci in the Baird’s pocket gopher (Geomys breviceps) and cross-amplification in other gopher species. Conservation Genet Resour 4, 467–469 (2012). https://doi.org/10.1007/s12686-011-9576-3

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  • DOI: https://doi.org/10.1007/s12686-011-9576-3

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