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Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

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Abstract

We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.

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Acknowledgements

This work was supported by funds from Pennsylvania State University Department of Biology. Permits were provided by the Alabama Department of Conservation and Natural Resources (# 3568, #4059, #4453), Arkansas Game and Fish Commission (# 031520061, #030220075, #022520081), Mississippi Department of Wildlife, Fisheries and Parks (to T. Langkilde), US Fish and Wildlife Service (# 43620-NOX-2006-02), and USDA Forest Service (# 2710 and SO-FW-FY06-08). The Institutional Animal Care and Use Committee at Yale University (#2006-11020) and Penn State University (#27696) approved this research. Tissue collection was assisted by Nisha Ligon and Katherine Boronow. We would also like to thank Tara Fulton for lab guidance. Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.

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This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.

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Authors and Affiliations

  1. Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA

    Stacey L. Lance & Travis C. Glenn

  2. Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC, 29802, USA

    Stacey L. Lance & Cris Hagen

  3. Department of Biology, The Pennsylvania State University, 208 Mueller Lab, University Park, PA, 16802, USA

    Nicole A. Freidenfelds & Tracy Langkilde

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  1. Stacey L. Lance
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  2. Cris Hagen
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  3. Travis C. Glenn
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  4. Nicole A. Freidenfelds
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  5. Tracy Langkilde
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Correspondence to Tracy Langkilde.

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Lance, S.L., Hagen, C., Glenn, T.C. et al. Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus . Conservation Genet Resour 1, 233–236 (2009). https://doi.org/10.1007/s12686-009-9057-0

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  • DOI: https://doi.org/10.1007/s12686-009-9057-0

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