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Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds

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Abstract

We used proteomics analysis to generate the profiles of proteins in the endosperm and embryo of common buckwheat grains. These differentially expressed proteins are potentially involved in seed metabolism. Extractions were done by trichloroacetic acid (TCA) precipitation. The resulting proteins were separated using SDS-PAGE coupled to LC-ESI-Q/TOF-MS/MS. This allowed us to detect and identify 67 proteins with isoforms, making this the most inclusive protein profile. The proteins were determined to be functionally involved in the central metabolic pathway of the seed, with metabolic interest being reflected in the occurrence of a tissue-specific enzyme balance. For a case in point, we found a tissue-specific and subcellular compartment-specific isoform of granule-bound starch synthase 1 in the chloroplast/amyloplast. This provided proteomic verification of the presence of a distinct regulatory mechanism for the biosynthesis of glycan and starch, which produce amylase and amylopectin. Furthermore, several previously characterized allergenic proteins such as 11S and 13S globulin seed storage protein were acknowledged in our seed samples, thus representing the potential for proteomics techniques that survey food sources for any incidence of allergens. This protein profile of common buckwheat grain is a new avenue for understanding its seed physiology in dormant stage as well as suggesting commercial applications for the buckwheat industry as buckwheat flour.

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Abbreviations

LC:

Liquid chromatography

PAGE:

Polyacrylamide gel electrophoresis

PIR:

Protein information resource

Q/TOF:

Quadruple/time of flight

SDS:

Sodium dodecyl sulfate

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Acknowledgements

This research was supported by a grant from Green Post-BI (2010 R&D-02) Chungbuk Green Industry Promotion Agency, Chungbuk, Korea, to S. H. Woo and partially funded by K-Mep Project (T30110) of Korea Basic Science Institute, Korea, to J. S. Choi.

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Authors and Affiliations

  1. Department of Crop Science, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk, 361-763, South Korea

    Abu Hena Mostafa Kamal, Ill-Dong Jang, Da-Eun Kim & Sun-Hee Woo

  2. National Agriculture and Food Research Organization for Hokkaido Region, Hokkaido, 082-0071, Japan

    Tatsuro Suzuki

  3. Department of Agricultural Chemistry, Chungbuk National University, Cheong-ju, 361-763, South Korea

    Keun-Yook Chung

  4. Division of Life Science, Korea Basic Science Institute, Daejeon, 305-333, South Korea

    Jong-Soon Choi

  5. Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, South Korea

    Jong-Soon Choi

  6. Department of Industrial Plant and Technology, Chungbuk National University, Cheong-ju, 361-763, South Korea

    Moon-Soon Lee

  7. Department of Plant Biotechnology, Kangwon National University, Chuncheon, 200-701, South Korea

    Cheol-Ho Park

  8. Department of Applied Botany, Chungnam National University, Daejeon, 305-764, South Korea

    Sang-Un Park

  9. Goesan Agricultural Technology and Extension Center, Cheong-ju, Chungbuk, 367-863, South Korea

    Seong Hee Lee

  10. Department of Food Science and Technology, Chungbuk National University, Cheong-ju, 361-763, South Korea

    Heon Sang Jeong

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  1. Abu Hena Mostafa Kamal
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  2. Ill-Dong Jang
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Correspondence to Sun-Hee Woo.

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Kamal, A.H.M., Jang, ID., Kim, DE. et al. Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds. J. Plant Biol. 54, 81–91 (2011). https://doi.org/10.1007/s12374-010-9143-6

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