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Discovery of Transcription Factors Novel to Mouse Cerebellar Granule Cell Development Through Laser-Capture Microdissection

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Abstract

Laser-capture microdissection was used to isolate external germinal layer tissue from three developmental periods of mouse cerebellar development: embryonic days 13, 15, and 18. The cerebellar granule cell-enriched mRNA library was generated with next-generation sequencing using the Helicos technology. Our objective was to discover transcriptional regulators that could be important for the development of cerebellar granule cells—the most numerous neuron in the central nervous system. Through differential expression analysis, we have identified 82 differentially expressed transcription factors (TFs) from a total of 1311 differentially expressed genes. In addition, with TF-binding sequence analysis, we have identified 46 TF candidates that could be key regulators responsible for the variation in the granule cell transcriptome between developmental stages. Altogether, we identified 125 potential TFs (82 from differential expression analysis, 46 from motif analysis with 3 overlaps in the two sets). From this gene set, 37 TFs are considered novel due to the lack of previous knowledge about their roles in cerebellar development. The results from transcriptome-wide analyses were validated with existing online databases, qRT-PCR, and in situ hybridization. This study provides an initial insight into the TFs of cerebellar granule cells that might be important for development and provide valuable information for further functional studies on these transcriptional regulators.

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Acknowledgements

We thank J. Yeung, J. Cairns, S. Tremblay, A. Poon, and J. Wilking for the support and suggestions on experimental design and manuscript preparation. We thank F. Lucero Villegas for animal management. We thank B. Lin, M. Larouche, D. Rains, and J. Boyle for technical support.

Funding

We thank National Institutes of Health, Natural Sciences and Engineering Research Council of Canada, NeuroDevNet, FANTOM OMICS Group, and University of British Columbia for funding.

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Authors and Affiliations

  1. Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, Department of Medical Genetics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4, Canada

    Peter G. Y. Zhang, Joanna Yeung, Ishita Gupta, Miguel Ramirez, Thomas Ha, Douglas J. Swanson & Dan Goldowitz

  2. RIKEN Omics Science Center (OSC), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan

    Sayaka Nagao-Sato, Masayoshi Itoh, Hideya Kawaji, Timo Lassmann, Carsten O. Daub, Erik Arner, Michiel de Hoon, Piero Carninci, Alistair R. R. Forrest & Yoshihide Hayashizaki

  3. RIKEN Center for Life Science Technologies, Yokohama, Japan

    Sayaka Nagao-Sato, Masayoshi Itoh, Hideya Kawaji, Timo Lassmann, Carsten O. Daub, Erik Arner, Michiel de Hoon, Piero Carninci & Alistair R. R. Forrest

  4. RIKEN Preventive Medicine and Diagnosis Innovation Program, Wako, Japan

    Masayoshi Itoh & Yoshihide Hayashizaki

  5. Telethon Kids Institute, The University of Western Australia, 100 Roberts Road, Subiaco, Subiaco, WA, 6008, Australia

    Timo Lassmann

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  1. Peter G. Y. Zhang
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  2. Joanna Yeung
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Correspondence to Dan Goldowitz.

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All experimentation with animals was under an approved Canadian Council on Animal Care research protocol (A12-0190).

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Zhang, P.G.Y., Yeung, J., Gupta, I. et al. Discovery of Transcription Factors Novel to Mouse Cerebellar Granule Cell Development Through Laser-Capture Microdissection. Cerebellum 17, 308–325 (2018). https://doi.org/10.1007/s12311-017-0912-3

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