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Comparative study of L-phenylalanine production in the growing and stationary phases during high cell density cultivation of an auxotrophic Escherichia coli

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Abstract

High cell density cultivation was investigated for L-phenylalanine (L-Phe) production by an L-tyrosine (L-Tyr) auxotrophic Escherichia coli WSH-BR165 (pAPB03). Dual exponential feeding of L-Tyr and glucose was adopted to achieve high cell density cultivation. The control was completed without dual feeding. The processes where dual feeding and induction were commenced together and those where induction began after dual feeding were studied and compared. The results indicated that the former dual feeding mode was most favorable for enhanced L-Phe production. With an optimal specific growth rate of 0.09/h during the dual exponential feeding period, the maximum dry cell weight reached 43.16 g/L (3.04 times that of the control) with a final L-Phe titer of 44.53 g/L (1.06 times that of the control) and an L-Phe productivity of 1.484 g/L/h (1.69 times that of the control). High cell density cultivation via the feeding of L-Tyr and glucose exponentially after the induction point proved to be an efficient approach to enhance L-Phe production.

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References

  1. Bongaerts, J., M. Kramer, U. Muller, L. Raeven, and M. Wubbolts (2001) Metabolic engineering for microbial production of aromatic amino acids and derived compounds. Metab. Eng. 3: 289–300.

    Article  CAS  Google Scholar 

  2. Shu, C. H. and C. C. Liao (2002) Optimization of L-phenylalanine production of Corynebacterium glutamicum under product feedback inhibition by elevated oxygen transfer rate. Biotechnol. Bioeng. 77: 131–141.

    Article  CAS  Google Scholar 

  3. Leuchtenberger, W., K. Huthmacher, and K. Drauz (2005) Biotechnological production of amino acids and derivatives: Current status and prospects. Appl. Microbiol. Biotechnol. 69: 1–8.

    Article  CAS  Google Scholar 

  4. Sprenger, G. (2007) Aromatic amino acids. pp. 93–127. In: Wendisch, V. F. (ed.). Amino acid biosynthesis pathways regulation and metabolic engineering. Springer, Berlin, Heidelberg.

    Chapter  Google Scholar 

  5. Ozcelik, I. S., P. Calik, G. Calik, and T. H. Ozdamar (2004) Metabolic engineering of aromatic group amino acid pathway in Bacillus subtilis for L-phenylalanine production. Chem. Eng. Sci. 59: 5019–5026.

    Article  Google Scholar 

  6. Gerigk, M., R. Bujnicki, E. Ganpo-Nkwenkwa, J. Bongaerts, G. Sprenger, and R. Takors (2002) Process control for enhanced Lphenylalanine production using different recombinant Escherichia coli strains. Biotechnol. Bioeng. 80: 746–754.

    Article  CAS  Google Scholar 

  7. Takors, R., M. Gerigk, H. Paschold, and C. Wandrey (2001) Principal-component analysis for microbial L-phenylalanine production. Bioproc. Biosyst. Eng. 24: 93–99.

    Article  CAS  Google Scholar 

  8. Hwang, S. O., G. H. Gil, Y. J. Cho, K. R. Kang, J. H. Lee, and J. C. Bae (1985) The fermentation process for L-phenylalanine production using an auxotrophic regulatory mutant of Escherichia coli. Appl. Microbiol. Biotechnol. 22: 108–113.

    Article  CAS  Google Scholar 

  9. Förberg, C. and L. Häggström (1988) Phenylalanine production from a rec Escherichia coli-strain in fed-batch culture. J. Biotechnol. 8: 291–300.

    Article  Google Scholar 

  10. Takagi, M., Y. Nishio, G. Oh, and T. Yoshida (1996) Control of L-phenylalanine production by dual feeding of glucose and Ltyrosine. Biotechnol. Bioeng. 52: 653–660.

    Article  CAS  Google Scholar 

  11. Zhou, H. Y., X. Y. Liao, T. W. Wang, G. C. Du, and J. Chen (2010) Enhanced L-phenylalanine biosynthesis by co-expression of pheA fbr and aroF wt. Bioresour. Technol. 101: 4151–4156.

    Article  CAS  Google Scholar 

  12. Henderson, J. W., R. D. Ricker, B. A. Bidlingmeyer, and C. Woodward (2000) Rapid, accurate, sensitive, and reproducible HPLC analysis of amino acids. (Agilent App Note 5980-1193E). Agilent Technologies, USA.

    Google Scholar 

  13. Yakandawala, N., T. Romeo, A. D. Friesen, and S. Madhyastha (2008) Metabolic engineering of Escherichia coli to enhance phenylalanine production. Appl. Microbiol. Biotechnol. 78: 283–291.

    Article  CAS  Google Scholar 

  14. Luo, Y. E., D. D. Fan, L. A. Shang, H. J. Shi, X. X. Ma, Y. Mi, and G. F. Zhao (2008) Analysis of metabolic flux in Escherichia coli expressing human-like collagen in fed-batch culture. Biotechnol. Lett. 30: 637–643.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, Wuxi, 214-122, China

    Jing Wang, Long Liu, Haiyan Zhou, Jianghua Li & Guocheng Du

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214-122, China

    Jing Wang, Long Liu, Haiyan Zhou, Jianghua Li & Guocheng Du

  3. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214-122, China

    Jian Chen

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  1. Jing Wang
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  2. Long Liu
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  3. Haiyan Zhou
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  4. Jianghua Li
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  5. Guocheng Du
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Correspondence to Long Liu or Guocheng Du.

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Wang, J., Liu, L., Zhou, H. et al. Comparative study of L-phenylalanine production in the growing and stationary phases during high cell density cultivation of an auxotrophic Escherichia coli . Biotechnol Bioproc E 16, 916–922 (2011). https://doi.org/10.1007/s12257-011-0135-2

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  • DOI: https://doi.org/10.1007/s12257-011-0135-2

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