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Enzymatic transformation of 2-O-α-D-glucopyranosyl-L-ascorbic acid by α-cyclodextrin glucanotransferase from recombinant Escherichia coli

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Abstract

The study aimed to produce 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) via the transglycosylation reaction by α-cyclodextrin glucanotransferase (α- CGTase) from recombinant Escherichia coli with L-ascorbic acid (AA) and β-cyclodextrin (β-CD) as the substrates. Liquid chromatography-tandem mass spectrometry analysis was conducted for AA-2G identification, and the glucoamylase treatment was carried out to produce AA-2G from AA-2-oilgosaccharides. The optimal temperature and pH for the enzymatic AA-2G production were 37°C and 5.5, respectively, and the optimal α-CGTase concentration and substrate mass ratio (AA:β-CD) for AA-2G synthesis were 160 U/mL and 1:1, respectively. At these optimal process conditions, maximal AA-2G production reached 13 g/L. This is the first report regarding the process optimization of enzymatic AA-2G production by α-CGTase from recombinant E. coli. The results may be useful for the industrial scale production of AA-2G.

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214-122, China

    Zichen Zhang, Jianghua Li, Long Liu, Zhaozhe Hua & Guocheng Du

  2. School of Biotechnology, Jiangnan University, Wuxi, 214-122, China

    Zichen Zhang, Jianghua Li, Long Liu & Zhaozhe Hua

  3. Institute of Information Technology, Jiangnan University, Wuxi, 214-122, China

    Jun Sun

  4. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214-122, China

    Jian Chen

Authors
  1. Zichen Zhang
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  2. Jianghua Li
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  3. Long Liu
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  4. Jun Sun
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  5. Zhaozhe Hua
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  6. Guocheng Du
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  7. Jian Chen
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Correspondence to Long Liu.

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Both the author’s equally contributed.

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Zhang, Z., Li, J., Liu, L. et al. Enzymatic transformation of 2-O-α-D-glucopyranosyl-L-ascorbic acid by α-cyclodextrin glucanotransferase from recombinant Escherichia coli . Biotechnol Bioproc E 16, 107–113 (2011). https://doi.org/10.1007/s12257-010-0161-5

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  • DOI: https://doi.org/10.1007/s12257-010-0161-5

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