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Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis

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Abstract

Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seoul, 136-713, Korea

    Joon-Ryeol Yang & Min-Kyu Oh

  2. School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Korea

    Eunjung Song & Byung-Gee Kim

  3. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Eung-Soo Kim

  4. Department of Pharmaceutical Engineering, SunMoon University, Asan, 336-708, Korea

    Jae-Kyung Sohng

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  1. Joon-Ryeol Yang
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  2. Eunjung Song
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  4. Eung-Soo Kim
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Correspondence to Min-Kyu Oh.

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Yang, JR., Song, E., Kim, BG. et al. Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis. Biotechnol Bioproc E 13, 738–744 (2008). https://doi.org/10.1007/s12257-008-0114-4

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  • DOI: https://doi.org/10.1007/s12257-008-0114-4

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