Abstract
This review describes the knee meniscus and its diverse cell populations. Situated between the femur and tibia, the meniscus acts to transmit loads within the knee while maintaining joint stability. Not only does this tissue display complex geometry and anatomy, its cellular profile ranges from fibroblast-like to chondrocyte-like. When the tissue first begins to develop in the body, its cells are similar in shape and morphology, but as it matures, these cells take on distinct characteristics. The spindle-shaped cells of the outer meniscus are well-suited to maintaining a fibrous extracellular matrix rich in collagen type I. The round, inner meniscus cells produce both collagen types I and II, and glycosaminoglycans, giving rise to a hyaline-like inner portion of the tissue. Cells intermediately located display characteristics of both cell types. Fibrochondrocytes are also known to be highly dependent on mechanical stimulation to maintain healthy tissue, and display regional variation in response to different biomolecular cues. Investigating this cell population under a variety of conditions can lead to a better understanding of the pathophysiology and regenerative processes of the meniscus.
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References
Ahluwalia, S., M. Fehm, M. M. Murray, S. D. Martin, and M. Spector. Distribution of smooth muscle actin-containing cells in the human meniscus. J. Orthop. Res. 19(4):659–664, 2001.
Aufderheide, A. C., and K. A. Athanasiou. Assessment of a bovine co-culture, scaffold-free method for growing meniscus-shaped constructs. Tissue Eng. 13(9):2195–2205, 2007.
Bhargava, M. M., E. T. Attia, G. A. Murrell, M. M. Dolan, R. F. Warren, and J. A. Hannafin. The effect of cytokines on the proliferation and migration of bovine meniscal cells. Am. J. Sports Med. 27(5):636–643, 1999.
Brindle, T., J. Nyland, and D. L. Johnson. The meniscus: review of basic principles with application to surgery and rehabilitation. J. Athl. Train. 36(2):160–169, 2001.
Cao, M., M. Stefanovic-Racic, H. I. Georgescu, L. A. Miller, and C. H. Evans. Generation of nitric oxide by lapine meniscal cells and its effect on matrix metabolism: stimulation of collagen production by arginine. J. Orthop. Res. 16(1):104–111, 1998.
Cheung, H. S. Distribution of type i, ii, iii and v in the pepsin solubilized collagens in bovine menisci. Connect. Tissue Res. 16(4):343–356, 1987.
Clark, C. R., and J. A. Ogden. Development of the menisci of the human knee joint. Morphological changes and their potential role in childhood meniscal injury. J. Bone Joint Surg. Am. 65(4):538–547, 1983.
Collier, S., and P. Ghosh. Effects of transforming growth factor beta on proteoglycan synthesis by cell and explant cultures derived from the knee joint meniscus. Osteoarthr. Cartil. 3(2):127–138, 1995.
Djurasovic, M., J. W. Aldridge, R. Grumbles, M. P. Rosenwasser, D. Howell, and A. Ratcliffe. Knee joint immobilization decreases aggrecan gene expression in the meniscus. Am. J. Sports Med. 26(3):460–466, 1998.
Fink, C., B. Fermor, J. B. Weinberg, D. S. Pisetsky, M. A. Misukonis, and F. Guilak. The effect of dynamic mechanical compression on nitric oxide production in the meniscus. Osteoarthr. Cartil. 9(5):481–487, 2001.
Gemmiti, C. V., and R. E. Guldberg. Fluid flow increases type ii collagen deposition and tensile mechanical properties in bioreactor-grown tissue-engineered cartilage. Tissue Eng. 12(3):469–479, 2006.
Ghadially, F. N., I. Thomas, N. Yong, and J. M. Lalonde. Ultrastructure of rabbit semilunar cartilages. J. Anat. 125(Pt 3):499–517, 1978.
Greis, P. E., D. D. Bardana, M. C. Holmstrom, and R. T. Burks. Meniscal injury: I. Basic science and evaluation. J. Am. Acad. Orthop. Surg. 10(3):168–176, 2002.
Gruber, H. E., D. Mauerhan, Y. Chow, J. A. Ingram, H. J. Norton, E. N. Hanley, Jr., and Y. Sun. Three-dimensional culture of human meniscal cells: extracellular matrix and proteoglycan production. BMC Biotechnol. 8:54, 2008.
Gunja, N. J., R. K. Uthamanthil, and K. A. Athanasiou. Effects of tgf-beta1 and hydrostatic pressure on meniscus cell-seeded scaffolds. Biomaterials 30(4):565–573, 2009.
Hellio Le Graverand, M. P., Y. Ou, T. Schield-Yee, L. Barclay, D. Hart, T. Natsume, and J. B. Rattner. The cells of the rabbit meniscus: their arrangement, interrelationship, morphological variations and cytoarchitecture. J. Anat. 198(Pt 5):525–535, 2001.
Hoben, G. M., and K. A. Athanasiou. Creating a spectrum of fibrocartilages through different cell sources and biochemical stimuli. Biotechnol. Bioeng. 100(3):587–598, 2008.
Kambic, H. E., and C. A. McDevitt. Spatial organization of types i and ii collagen in the canine meniscus. J. Orthop. Res. 23(1):142–149, 2005.
Marsano, A., D. Wendt, T. M. Quinn, T. J. Sims, J. Farhadi, M. Jakob, M. Heberer, and I. Martin. Bi-zonal cartilaginous tissues engineered in a rotary cell culture system. Biorheology 43(3–4):553–560, 2006.
McAlinden, A., J. Dudhia, M. C. Bolton, P. Lorenzo, D. Heinegard, and M. T. Bayliss. Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage. Osteoarthr. Cartil. 9(1):33–41, 2001.
Melrose, J., S. Smith, M. Cake, R. Read, and J. Whitelock. Comparative spatial and temporal localisation of perlecan, aggrecan and type i, ii and iv collagen in the ovine meniscus: an ageing study. Histochem. Cell Biol. 124(3–4):225–235, 2005.
Mikic, B., T. L. Johnson, A. B. Chhabra, B. J. Schalet, M. Wong, and E. B. Hunziker. Differential effects of embryonic immobilization on the development of fibrocartilaginous skeletal elements. J. Rehabil. Res. Dev. 37(2):127–133, 2000.
Miller, R. R., and P. A. Rydell. Primary culture of microvascular endothelial cells from canine meniscus. J. Orthop. Res. 11(6):907–911, 1993.
Moon, M. S., J. M. Kim, and I. Y. Ok. The normal and regenerated meniscus in rabbits. Morphologic and histologic studies. Clin. Orthop. Relat. Res. 182:264–269, 1984.
Mueller, S. M., T. O. Schneider, S. Shortkroff, H. A. Breinan, and M. Spector. Alpha-smooth muscle actin and contractile behavior of bovine meniscus cells seeded in type i and type ii collagen-gag matrices. J. Biomed. Mater. Res. 45(3):157–166, 1999.
Mueller, S. M., S. Shortkroff, T. O. Schneider, H. A. Breinan, I. V. Yannas, and M. Spector. Meniscus cells seeded in type i and type ii collagen-gag matrices in vitro. Biomaterials 20(8):701–709, 1999.
Nakata, K., K. Shino, M. Hamada, T. Mae, T. Miyama, H. Shinjo, S. Horibe, K. Tada, T. Ochi, and H. Yoshikawa. Human meniscus cell: characterization of the primary culture and use for tissue engineering. Clin. Orthop. Relat. Res. 391(Suppl):S208–218, 2001.
Natsu-Ume, T., T. Majima, C. Reno, N. G. Shrive, C. B. Frank, and D. A. Hart. Menisci of the rabbit knee require mechanical loading to maintain homeostasis: Cyclic hydrostatic compression in vitro prevents derepression of catabolic genes. J. Orthop. Sci. 10(4):396–405, 2005.
Neves, A. A., N. Medcalf, and K. M. Brindle. Tissue engineering of meniscal cartilage using perfusion culture. Ann. N Y Acad. Sci. 961:352–355, 2002.
O’Reilly, M. S., T. Boehm, Y. Shing, N. Fukai, G. Vasios, W. S. Lane, E. Flynn, J. R. Birkhead, B. R. Olsen, and J. Folkman. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88(2):277–285, 1997.
Ochi, K., Y. Daigo, T. Katagiri, A. Saito-Hisaminato, T. Tsunoda, Y. Toyama, H. Matsumoto, and Y. Nakamura. Expression profiles of two types of human knee-joint cartilage. J. Hum.Genet. 48(4):177–182, 2003.
Ochi, M., T. Kanda, Y. Sumen, and Y. Ikuta. Changes in the permeability and histologic findings of rabbit menisci after immobilization. Clin. Orthop. Relat. Res. 334:305–315, 1997.
Pangborn, C. A., and K. A. Athanasiou. Effects of growth factors on meniscal fibrochondrocytes. Tissue Eng. 11(7–8):1141–1148, 2005.
Pangborn, C. A., and K. A. Athanasiou. Growth factors and fibrochondrocytes in scaffolds. J. Orthop. Res. 23(5):1184–1190, 2005.
Park, L. S., J. A. Jacobson, D. A. Jamadar, E. Caoili, M. Kalume-Brigido, and E. Wojtys. Posterior horn lateral meniscal tears simulating meniscofemoral ligament attachment in the setting of ACL tear: MRI findings. Skeletal Radiol. 36(5):399–403, 2007.
Pufe, T., W. J. Petersen, N. Miosge, M. B. Goldring, R. Mentlein, D. J. Varoga, and B. N. Tillmann. Endostatin/collagen xviii—an inhibitor of angiogenesis—is expressed in cartilage and fibrocartilage. Matrix Biol. 23(5):267–276, 2004.
Spindler, K. P., C. E. Mayes, R. R. Miller, A. K. Imro, and J. M. Davidson. Regional mitogenic response of the meniscus to platelet-derived growth factor (pdgf-ab). J. Orthop. Res. 13(2):201–207, 1995.
Tanaka, T., K. Fujii, and Y. Kumagae. Comparison of biochemical characteristics of cultured fibrochondrocytes isolated from the inner and outer regions of human meniscus. Knee Surg. Sports Traumatol. Arthrosc. 7(2):75–80, 1999.
Upton, M. L., J. Chen, F. Guilak, and L. A. Setton. Differential effects of static and dynamic compression on meniscal cell gene expression. J. Orthop. Res. 21(6):963–969, 2003.
Upton, M. L., F. Guilak, T. A. Laursen, and L. A. Setton. Finite element modeling predictions of region-specific cell-matrix mechanics in the meniscus. Biomech. Model Mechanobiol. 5(2–3):140–149, 2006.
Upton, M. L., A. Hennerbichler, B. Fermor, F. Guilak, J. B. Weinberg, and L. A. Setton. Biaxial strain effects on cells from the inner and outer regions of the meniscus. Connect. Tissue Res. 47(4):207–214, 2006.
Verdonk, P. C., R. G. Forsyth, J. Wang, K. F. Almqvist, R. Verdonk, E. M. Veys, and G. Verbruggen. Characterisation of human knee meniscus cell phenotype. Osteoarthr. Cartil. 13(7):548–560, 2005.
Webber, R. J., M. G. Harris, and A. J. Hough, Jr. Cell culture of rabbit meniscal fibrochondrocytes: proliferative and synthetic response to growth factors and ascorbate. J. Orthop. Res. 3(1):36–42, 1985.
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The authors would like to acknowledge NIAMS R01 AR 47839-2 for funding this work.
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Sanchez-Adams, J., Athanasiou, K.A. The Knee Meniscus: A Complex Tissue of Diverse Cells. Cel. Mol. Bioeng. 2, 332–340 (2009). https://doi.org/10.1007/s12195-009-0066-6
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DOI: https://doi.org/10.1007/s12195-009-0066-6