Abstract
Pesticide residues are detected in food items globally at levels that may cause health problems. Paper-based analytical devices have emerged as an alternative low-cost and field-based tools for determining target analytes including the pesticide residues. However, the paper devices with colorimetric detection system are limited by lower sensitivity due to their inability to record wavelength specific signal from the assays when image analysis detection method is used. In this paper, we report a low-cost analytical method that combines paper-analytical platform with a do-it-yourself desktop spectrometer to determine pesticide residues in vegetables and fruits. The pesticide determination method utilizes acetylcholinesterase enzyme inhibition assay on paper platform for signal generation. The signal of the assay was then measured using a spectrometer made from locally available components. The enzyme reaction parameters were optimized using multivariate optimization methodology to obtain better assay signal. The average repeatability of the assay method was 5.7% RSD. The combined method responded to the concentration of dichlorvos pesticide with a linear range from 0.01 to 0.25 mg/kg and detection limit of 0.023 mg/kg using only 4 μL of reagents and sample. As the enzyme inhibition assay used in this work is not able to discriminate individual pesticides, it can be used as a low-cost method for initial screening of pesticide residues in food samples in field settings.
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References
Apilux A, Isarankura-Na-Ayudhya C, Tantimongcolwat T, Prachayasittikul V (2015) Paper-based acetylcholinesterase inhibition assay combining a wet system for organophosphate and carbamate pesticides detection. EXCLI J 14:307–319. https://doi.org/10.17179/excli2014-684
Badawy ME, El-Aswad AF (2014) Bioactive paper sensor based on the acetylcholinesterase for the rapid detection of organophosphate and carbamate pesticides. Int J Anal Chem 2014:536823–536828. https://doi.org/10.1155/2014/536823
Bakırcı GT, Yaman Acay DB, Bakırcı F, Ötleş S (2014) Pesticide residues in fruits and vegetables from the Aegean region, Turkey. Food Chem 160:379–392. https://doi.org/10.1016/j.foodchem.2014.02.051
Bass JJ, Wilkinson DJ, Rankin D, Phillips BE, Szewczyk NJ, Smith K, Atherton PJ (2017) An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports 27(1):4–25. https://doi.org/10.1111/sms.12702
Bhandari G, Zomer P, Atreya K, Mol HGJ, Yang X, Geissen V (2019) Pesticide residues in Nepalese vegetables and potential health risks. Environ Res 172:511–521. https://doi.org/10.1016/j.envres.2019.03.002
Caldas LFS, Francisco BBA, Netto ADP, Cassella RJ (2011) Multivariate optimization of a spectrophotometric method for copper determination in Brazilian sugar-cane spirits using the Doehlert design. Microchem J 99(1):118–124. https://doi.org/10.1016/j.microc.2011.04.008
Caldas LFS, de Paula CER, Brum DM, Cassella RJ (2013) Application of a four-variables Doehlert design for the multivariate optimization of copper determination in petroleum-derived insulating oils by GFAAS employing the dilute-and-shot approach. Fuel 105:503–511. https://doi.org/10.1016/j.fuel.2012.10.026
Cheng Y, Prusoff WH (1973) Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem Pharmacol 22(23):3099–3108. https://doi.org/10.1016/0006-2952(73)90196-2
Commission E, (2005) Regulation (EC) no 396/2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending council directive 91/414/EEC. 396/2005 E. commission. 91/414/EEC
Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70–77. https://doi.org/10.1016/0003-9861(59)90090-6
Ferreira SL, Bezerra MA, dos Santos WN, Neto BB (2003) Application of Doehlert designs for optimisation of an on-line preconcentration system for copper determination by flame atomic absorption spectrometry. Talanta 61(3):295–303. https://doi.org/10.1016/S0039-9140(03)00277-7
Ferreira SL, Dos Santos WN, Quintella CM, Neto BB, Bosque-Sendra JM (2004) Doehlert matrix: a chemometric tool for analytical chemistry—review. Talanta 63(4):1061–1067. https://doi.org/10.1016/j.talanta.2004.01.015
Gallibu C, Gallibu C, Avoundjian A, Gomez FA (2016) Easily fabricated microfluidic devices using permanent marker inks for enzyme assays. Micromachines 7(1):6. https://doi.org/10.3390/mi7010006
Gibney E (2016) Open-hardware’pioneers push for low-cost lab kit 531, 147
Giri B (2016) A perspective on the sensitivity of paper-analytical devices for bioanalysis. J Appl Bioanal 2(1):6–9. https://doi.org/10.17145/jab.16.002
Hanot V, Goscinny S, Deridder M (2015) A simple multi-residue method for the determination of pesticides in fruits and vegetables using a methanolic extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry: optimization and extension of scope. J Chromatogr A 1384:53–66. https://doi.org/10.1016/j.chroma.2015.01.040
Hossain SM, Luckham RE, McFadden MJ, Brennan JD (2009) Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Anal Chem 81(21):9055–9064
Jallow MFA, Awadh DG, Albaho MS, Devi VY, Ahmad N (2017) Monitoring of pesticide residues in commonly used fruits and vegetables in Kuwait. Int J Environ Res Public Health 14(8):833. https://doi.org/10.3390/ijerph14080833
Kim KH, Kabir E, Jahan SA (2017) Exposure to pesticides and the associated human health effects. Sci Total Environ 575:525–535. https://doi.org/10.1016/j.scitotenv.2016.09.009
Krstić DZ, Colović M, Kralj MB, Franko M, Krinulović K, Trebse P, Vasić V (2008) Inhibition of AChE by malathion and some structurally similar compounds. J Enzyme Inhib Med Chem 23(4):562–573. https://doi.org/10.1080/14756360701632031
Levine MN, Raines RT (2011) Sensitive fluorogenic substrate for alkaline phosphatase. Anal Biochem 418(2):247–252. https://doi.org/10.1016/j.ab.2011.07.021
Luckham RE, Brennan JD (2010) Bioactive paper dipstick sensors for acetylcholinesterase inhibitors based on sol-gel/enzyme/gold nanoparticle composites. Analyst 135(8):2028–2035
Meng X, Schultz CW, Cui C, Li X, Yu HZ (2015) On-site chip-based colorimetric quantitation of organophosphorus pesticides using an office scanner. Sensors Actuators B Chem 215:577–583. https://doi.org/10.1016/j.snb.2015.04.011
Mettakoonpitak J, Boehle K, Nantaphol S, Teengam P, Adkins JA, Srisa-Art M, Henry CS (2016) Electrochemistry on paper-based analytical devices: a review. Electroanalysis 28(7):1420–1436. https://doi.org/10.1002/elan.201501143
Nouanthavong S, Nacapricha D, Henry CS, Sameenoi Y (2016) Pesticide analysis using nanoceria-coated paper-based devices as a detection platform. Analyst 141(5):1837–1846
Public Lab (2018) Desktop Spectrometry Kit 2.0. from https://publiclab.org/wiki/desktop-spectrometry-kit-3-0. Accessed 24 Dec 2019
Pundir CS, Chauhan N (2012) Acetylcholinesterase inhibition-based biosensors for pesticide determination: a review. Anal Biochem 429(1):19–31. https://doi.org/10.1016/j.ab.2012.06.025
Rathore HS, Nollet LM (2016) Handbook of pesticides: methods of pesticide residues analysis. CRC press
Senanayake N, Karalliedde L (1987) Neurotoxic effects of organophosphorus insecticides. An intermediate syndrome. N Engl J Med 316(13):761–763. https://doi.org/10.1056/NEJM198703263161301
Sharma N, Barstis T, Giri B (2018) Advances in paper-analytical methods for pharmaceutical analysis. Eur J Pharm Sci 111:46–56. https://doi.org/10.1016/j.ejps.2017.09.031
Skretteberg LG, Lyrån B, Holen B, Jansson A, Fohgelberg P, Siivinen K, Andersen JH, Jensen BH (2015) Pesticide residues in food of plant origin from Southeast Asia–a Nordic project. Food Control 51:225–235. https://doi.org/10.1016/j.foodcont.2014.11.008
Soriano S, Netto ADP, Cassella RJ (2007) Multivariate optimization of a microwave-assisted leaching procedure using dilute acid solutions, for FAAS determination of Cu, Fe, Mn, and Zn in multivitamin/multimineral supplements. Anal Bioanal Chem 387(3):1113–1120. https://doi.org/10.1007/s00216-006-0973-5
Spectral Workbench (n.d.) from https://spectralworkbench.org. Accessed 24 Dec 2019
Taha S, Rafat G, Aboshosha F, Mansour FR (2017) A simple homemade spectrophotometer. J Anal Chem 72(2):239–242. https://doi.org/10.1134/S1061934817020113
Tavener SJ, Thomas-Oates JE (2017) Build your own spectrophotometer. Educ Chem 44(5):151–154
Team RC (2017) R: a language and environment for statistical computing Vienna Austria, R Found Stat Comput . 3.4.2
Tomlin CD (2009) The pesticide manual: a world compendium, British Crop Production Council
Wilson MV, Wilson E (2016) Authentic performance in the instrumental analysis laboratory: building a visible spectrophotometer prototype. J Chem Educ 94(1):44–51. https://doi.org/10.1021/acs.jchemed.6b00515
Wu Y, Sun Y, Xiao F, Wu Z, Yu R (2017) Sensitive inkjet printing paper-based colormetric strips for acetylcholinesterase inhibitors with indoxyl acetate substrate. Talanta 162:174–179
Yadav IC, Devi NL, Syed JH, Cheng Z, Li J, Zhang G, Jones KC (2015) Current status of persistent organic pesticides residues in air, water, and soil, and their possible effect on neighboring countries: a comprehensive review of India. Sci Total Environ 511:123–137. https://doi.org/10.1016/j.scitotenv.2014.12.041
Yang Y, Noviana E, Nguyen MP, Geiss BJ, Dandy DS, Henry CS (2017) Paper-based microfluidic devices: emerging themes and applications. Anal Chem 89(1):71–91. https://doi.org/10.1021/acs.analchem.6b04581
Funding
This work was supported by NAS and USAID (AID-OAA-A-11-00012) through Partnerships for Enhanced Engagement in Research (PEER) grant. Preliminary work of this paper was supported by International Foundation for Science (IFS), Sweden, and Organization for the Prohibition of Chemical Weapons (OPCW), The Hague (Research Grant No. F/5777–1), and The World Academy of Sciences (TWAS), Italy (Research Grant No. 15–209 RG/CHE/AS_I – FR3240287080). Funding agencies were not involved in designing and implementing the research project and the paper do not necessarily reflect their views.
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Muna Fuyal declares that she has no conflict of interest. Basant Giri declares that he has no conflict of interest.
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Fuyal, M., Giri, B. A Combined System of Paper Device and Portable Spectrometer for the Detection of Pesticide Residues. Food Anal. Methods 13, 1492–1502 (2020). https://doi.org/10.1007/s12161-020-01770-y
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DOI: https://doi.org/10.1007/s12161-020-01770-y