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Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS)

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International Journal for Ion Mobility Spectrometry

Abstract

Matrix-assisted laser/desorption ionization (MALDI) time-of-flight mass spectrometry (TOF) has been investigated for use in the field of metabolomics; however, difficulties, mainly due to chemical interferences, are typically encountered. By coupling MALDI with ion mobility time-of-flight mass spectrometry (IMMS), isomers and isobars are resolved in mobility space reducing the chemical interference from matrix/background ions. MALDI-IMMS offers the advantages of high sensitivity, high throughput and low sample consumption. For this study, MALDI-IMMS was evaluated by monitoring metabolic changes in lymphatic fluid collected from fasting and fed rats. The number of metabolite features detected in the samples ranged between 1200 and 3400 depending on the duration between the feeding time and lymph sample collection. There were 747 metabolite features that were statistically analyzed by principal component analysis (PCA). From the 3-D score plots of PC1, PC2 and PC3 65 % of the original variation of the system was explained and the differences between the samples were demonstrated.

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Acknowledgements

Authors would like to acknowledge Andrea Choiniere for her help in peak alignment with the data. This project was supported in part by a research grant from Department of Health and Human Service: Public Health Services organization (Road Map Grant No. R21 DK070274). We would like to acknowledge the work performed by the Cincinnati Mouse Metabolic Phenotype Center support by DK 59630. Ionwerk’s J. Albert Schultz and Thomas Egan for instrumental and software help, NIDA contract # N44DA-3-7727and HHSN271200677593C, HHSN271200677563C for building the instrument used in this work, and National Institute on Drug Abuse Intramural program for funding to A.S. Woods laboratory.

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Authors and Affiliations

  1. Washington State University, Pullman, WA, 99164, USA

    Kimberly Kaplan, Prabha Dwivedi, William Siems & Herbert H. Hill Jr.

  2. NIDA IRP, NIH, Baltimore, MD, 21224, USA

    Shelley Jackson & Amina Woods

  3. University of Cincinnati, Cincinnati, OH, 45221, USA

    W. Sean Davidson, Qing Yang & Patrick Tso

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  1. Kimberly Kaplan
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  2. Shelley Jackson
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  3. Prabha Dwivedi
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  4. W. Sean Davidson
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  5. Qing Yang
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  6. Patrick Tso
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  7. William Siems
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  8. Amina Woods
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  9. Herbert H. Hill Jr.
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Correspondence to Herbert H. Hill Jr..

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Kaplan, K., Jackson, S., Dwivedi, P. et al. Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS). Int. J. Ion Mobil. Spec. 16, 177–184 (2013). https://doi.org/10.1007/s12127-012-0102-4

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  • DOI: https://doi.org/10.1007/s12127-012-0102-4

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