Abstract
Almost complete assignment of backbone 1H, 13C, 15N and side chain 13Cβ resonances for the immune-regulatory cytokine IL-10 is reported. The protein was overexpressed in Escherichia coli and was refolded from inclusion bodies. The point mutation C149Y was introduced to suppress incorrect disulfide bond formation and to improve protein refolding.
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Acknowledgments
We thank Sebastian Lanvermann (Technical University Dresden) who provided the IL-10 cDNA and Professors Stefan Berger (University of Leipzig) and Jochen Balbach (Martin Luther University Halle/Wittenberg) who kindly provided access to their NMR spectrometers. The study was supported by a grant from the Deutsche Forschungsgemeinschaft (Transregio 67, A6) and a grant from the Stipendienfonds der Chemischen Industrie e.V.
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Künze, G., Theisgen, S. & Huster, D. Backbone 1H, 15N, 13C and side chain 13Cβ NMR chemical shift assignment of murine interleukin-10. Biomol NMR Assign 8, 375–378 (2014). https://doi.org/10.1007/s12104-013-9521-3
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DOI: https://doi.org/10.1007/s12104-013-9521-3