Abstract
Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the 15N, 13C, and 1H chemical shift assignments of the intein from DNA polymerase II of Pyrococcus abyssi (Pab PolII intein), which has been recombinantly overexpressed and isotopically labeled. The NMR assignments of Pab PolII intein are essential for solution structure determination and protein dynamics study.
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Acknowledgments
The project described was supported by Award Number R01GM081408 (to C.W.) from the National Institute of General Medical Sciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health. We thank Sheela Rao for her experimental help.
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Liu, J., Du, Z., Albracht, C.D. et al. 1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein. Biomol NMR Assign 5, 233–235 (2011). https://doi.org/10.1007/s12104-011-9307-4
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DOI: https://doi.org/10.1007/s12104-011-9307-4