Abstract
GrxS14 is a monothiol Glutaredoxin (Grx) from Populus tremula × tremuloides, which has a CGFS active site. GrxS14 is located in the chloroplasts and has been found to occur ether as an apo form or as a holo form with a [2Fe-2S] cluster. The holo form contains two monomers of apo GrxS14 bridged by the iron sulphur center, in the presence of two external glutathione molecules (Bandyopadhyay et al. 2008). The NMR assignments of the GrxS14 are essential for its solution structure determination.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bandyopadhyay S, Gama F, Molina-Navarro MM, Gualberto JM, Claxton R, Naik SG, Huynh BH, Herrero E, Jacquot JP, Johnson MK, Rouhier N (2008) Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe–2S] clusters. EMBO J 27(7):1122–1133
Couturier J, Koh CS, Zaffagnini M, Winger AM, Gualberto JM, Corbier C, Decottignies P, Jacquot JP, Lemaire SD, Didierjean C, Rouhier N (2009) Structure-function relationship of the chloroplastic glutaredoxin s12 with an atypical wcsys active site. J Biol Chem 284(14):9299–9310
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) Nmrpipe: a multidimensional spectral processing system based on unix pipes. J Biomol NMR 6(3):277–293
Feng Y, Zhong N, Rouhier N, Hase T, Kusunoki M, Jacquot JP, Jin C, Xia B (2006) Structural insight into poplar glutaredoxin c1 with a bridging iron-sulfur cluster at the active site. Biochemistry 45(26):7998–8008
Johnson BA, Blevins RA (1994) Nmr view: a computer program for the visualization and analysis of nmr data. J Biomol NMR 4:603–614
Lillig CH, Berndt C, Holmgren A (2008) Glutaredoxin systems. Biochim Biophys Acta 1780(11):1304–1317
Markley JL, Bax A, Arata Y, Hilbers CW, Kaptein R, Sykes BD, Wright PE, Wuthrich K (1998) Recommendations for the presentation of nmr structures of proteins and nucleic acids. Iupac-iubmb-iupab inter-union task group on the standardization of data bases of protein and nucleic acid structures determined by nmr spectroscopy. J Biomol NMR 12(1):1–23
Noguera V, Walker O, Rouhier N, Jacquot JP, Krimm I, Lancelin JM (2005) Nmr reveals a novel glutaredoxin-glutaredoxin interaction interface. J Mol Biol 353(3):629–641
Rouhier N, Gelhaye E, Jacquot JP (2004) Plant glutaredoxins: still mysterious reducing systems. Cell Mol Life Sci 61(11):1266–1277
Rouhier N, Couturier J, Jacquot JP (2006) Genome-wide analysis of plant glutaredoxin systems. J Exp Bot 57(8):1685–1696
Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP (2007) Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe–2S] cluster in poplar glutaredoxin c1. Proc Natl Acad Sci U S A 104(18):7379–7384
Rouhier N, Lemaire SD, Jacquot JP (2008) The role of glutathione in photosynthetic organisms: emerging functions for glutaredoxins and glutathionylation. Annu Rev Plant Biol 59:143–166
Acknowledgments
All NMR experiments were carried out at the Beijing NMR Center. This research was supported by grant 2006DFA31210 from the International S&T Cooperation Program of Ministry of Science and Technology of China.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, L., Ren, X., Li, Y. et al. 1H, 13C, and 15N resonance assignments of reduced GrxS14 from Populus tremula × tremuloides . Biomol NMR Assign 5, 121–124 (2011). https://doi.org/10.1007/s12104-010-9282-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12104-010-9282-1