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Genome Sequencing Revealed the Biotechnological Potential of an Obligate Thermophile Geobacillus thermoleovorans Strain RL Isolated from Hot Water Spring

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Abstract

In the present study, we report the draft genome sequence of an obligate thermophile Geobacillus thermoleovorans strain RL isolated from Manikaran hot water spring located atop the Himalayan ranges, India. Strain RL grew optimally at 70 °C but not below 45 °C. The draft genome (3.39 Mb) obtained by Illumina sequencing contains 138 contigs with an average G + C content of 52.30%. RAST annotation showed that amino acid metabolism pathways were most dominant followed by carbohydrate metabolism. Genome-wide analysis using NCBI’s Prokaryotic Genome Annotation Pipeline revealed that strain RL encodes for a cocktail of industrially important hydrolytic enzymes glycoside hydrolase, α-and β-glucosidase, xylanase, amylase, neopullulanase, pullulanase and lipases required for white biotechnology. In addition, the presence of genes encoding green biocatalyst multicopper polyphenol oxidase (laccase) and an anticancer enzyme l-glutaminase reflects the significance of strain RL in gray and red biotechnology, respectively. Strain RL is a thermophilic multi-enzyme encoding bacterium which could be the source for the recombinant production of biotechnologically significant enzymes. In, addition whole cells of strain RL may be used in bioremediation studies.

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Acknowledgements

This work was supported by a Grant to AKS (SERB N-PDF (PDF/2016/002780). DNS acknowledge Council of Scientific and Industrial Research (CSIR) for providing financial support under CSIR-Senior Research Associate (Pool Scientist’s Scheme). US and VG acknowledge University Grant Commission (UGC) and CSIR, respectively for providing Senior Research Fellowship. CDR is grateful to Dr. Manoj Khanna, Principal, Ramjas College, University of Delhi for his constant support.

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Author notes

  1. Utkarsh Sood, Vipin Gupta & Rup Lal

    Present address: PhiXGen Private Limited, Gurugram, Haryana, 122001, India

  2. Durgesh Narain Singh, Utkarsh Sood and Amit Kumar Singh have contributed equally to this work.

Authors and Affiliations

  1. Department of Zoology, University of Delhi, Delhi, 110007, India

    Durgesh Narain Singh, Utkarsh Sood, Vipin Gupta, Mallikarjun Shakarad & Rup Lal

  2. Department of Biotechnology, Jamia Millia Islamia, New Delhi, Delhi, 110025, India

    Amit Kumar Singh

  3. Department of Zoology, Ramjas College, University of Delhi, Delhi, 110007, India

    Charu Dogra Rawat

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  1. Durgesh Narain Singh
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Singh, D.N., Sood, U., Singh, A.K. et al. Genome Sequencing Revealed the Biotechnological Potential of an Obligate Thermophile Geobacillus thermoleovorans Strain RL Isolated from Hot Water Spring. Indian J Microbiol 59, 351–355 (2019). https://doi.org/10.1007/s12088-019-00809-x

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