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Probiotic Bacillus amyloliquefaciens SC06 Prevents Bacterial Translocation in Weaned Mice

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Abstract

Probiotic is a preparation containing microorganisms that confers beneficial effect to the host. This work assessed whether oral administration of Bacillus amyloliquefaciens SC06 (Ba) could decrease bacterial translocation in weaned mice. Weaned C57BL/6 were randomly allocated into three groups: group I as the control group, group II were treated with 0.85 % NaCl. Group III was administered with probiotic Ba 1 × 109 CFU/day dissolved in 100 μl of 0.85 % NaCl for 30 days. Mice were then sacrificed, and tissue were cultured to determine bacterial translocation. Meanwhile, splenic CD4+T cells, CD8+T cells, B cells, and macrophages were analysised by FACS. Our results showed that probiotic Ba significantly reduced bacteria translocation compared with the control group and 0.85 % NaCl group (P < 0.05), lower levels of bacteria were detected in the MLN, liver, spleen, and kidney of mice. Moreover, significant increase in percentage and number of macrophages were observed in the spleen of Ba-treated mice compared with the control and 0.85 % NaCl groups. Together, these data indicated that Ba could decrease bacterial translocation in weaned mice. This effect seems to be correlated with the changes of macrophage numbers.

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Acknowledgments

We are grateful to Dr. Linrong Lu (Zhejiang University School of Medicine) for generous support and insightful guidance. This study was supported by the grants from Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110101110101).

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Authors and Affiliations

  1. College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Molecular Nutrition of Education of Ministry, Hangzhou, 310058, Zhejiang, China

    Jian Ji, Shenglan Hu & Weifen Li

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  1. Jian Ji
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  2. Shenglan Hu
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  3. Weifen Li
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Corresponding author

Correspondence to Weifen Li.

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Jian Ji and Shenglan Hu contributed equally to this work.

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Ji, J., Hu, S. & Li, W. Probiotic Bacillus amyloliquefaciens SC06 Prevents Bacterial Translocation in Weaned Mice. Indian J Microbiol 53, 323–328 (2013). https://doi.org/10.1007/s12088-013-0387-y

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  • DOI: https://doi.org/10.1007/s12088-013-0387-y

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