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Effect of concomitant progesterone administration or the effect of removal of estrogen capsule on changes caused by long-term estrogen treatment in pituitary VIP immunoreactivities

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Abstract

In the anterior pituitary besides the classical tropic hormones, peptides of a small molecular weight are also synthesized. One of them is the vasoactive intestinal polypeptide (VIP). VIP immunoreactivity is readily detected in human and monkey pituitaries; however, in the rat VIP immunoreactive cells were observed in about 50% of intact rats. In estrogen treated rats VIP immunoreactive cells were observed in the anterior pituitary of all animals. In this work, we have examined the effect of long-term sexual steroid treatments on the VIP immunoreactivity of the anterior pituitary using diethylstilbestrol (DES) or progesterone (P) filled capsules. The effectiveness of steroid treatments was tested by the measurement of plasma prolactin (PRL) level and by the appearance of prolactinoma. DES enhanced the plasma PRL level and 5 months later it induced prolactinomas, the concomitant P treatment prevented both the elevation of plasma PRL level and the formation of prolactinomas. These results indicated that there was enough steroid in the capsules. There was a positive correlation between the duration of DES influence and the number of VIP immunoreactive cells. Two months after the implantation of DES there was a considerable number of VIP cells in the anterior pituitary, and 5 months after implantation the number of VIP cells was greatly increased so as to form a VIP-oma. Concomitant implantation of P prevented the formation of VIP-oma. Two months after the implantation, the DES capsule was removed. Already 2 months after removal the number of VIP cells approximated to the control level. It has been concluded that P can prevent the undesired effect of DES not only on the PRL, but on the VIP immunoreactivity as well.

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Abbreviations

DES:

Diethylstilbestrol

E:

Estrogen

E2:

Estradiol

ECAP:

Empty capsule

ER:

Estrogen receptor

FSCs:

Folliculostellate cells

FSH:

Follicle stimulating hormone

ir:

Immunoreactive

LH:

Luteinizing hormone

P:

Progesterone

PR:

Progesterone receptor

PRL:

Prolactin

VIP:

Vasoactive intestinal polypeptide

References

  1. W.W. Morgan, R.W. Steger, M.S. Smith, A. Bartke, C.A. Sweeney, Endocrinology 116, 17–24 (1985)

    Article  CAS  PubMed  Google Scholar 

  2. S. Koike, M. Sakai, M. Muramatsu, Nucleic Acids Res. 15, 2499–2513 (1987)

    Article  CAS  PubMed  Google Scholar 

  3. G.G.J.M. Kuiper, E. Enmark, M. Pelto-Huikko, S. Nilsson, J.A. Gustaffsson, Proc. Natl. Acad. Sci. USA 93, 5925–5930 (1996)

    Article  CAS  PubMed  Google Scholar 

  4. M. Gonzales, R. Reyes, C. Damas, R. Alonso, A.R. Bello, Gen. Comp. Endocrinol. 155, 857–868 (2008)

    Article  Google Scholar 

  5. M.E. Wilson, R.H. Price Jr., R.J. Handa, Endocrinology 139, 5151–5156 (1998)

    Article  CAS  PubMed  Google Scholar 

  6. M. Szabó, S.M. Kilen, S.J. Nho, N.B. Schwartz, Biol. Reprod. 62, 95–102 (2000)

    Article  PubMed  Google Scholar 

  7. J.C. Garrido-Gracia, A. Gordon, C. Bellido, R. Aguilar, I.I. Barranco, Y. Millán, J.M. de Las Mulas, J.E. Sánchez-Criado, J. Endocrinol. 193, 107–119 (2007)

    Article  CAS  PubMed  Google Scholar 

  8. R.D. Brinton, R.F. Thompson, M.R. Roy, M. Baudry, Y.M. Wang, C.E. Finch, T.E. Morgan, C.J. Pike, W.J. Mack, F.Z. Stanczyk, J. Nilsen, Front. Neuroendocrinol. 29, 313–339 (2008)

    CAS  PubMed  Google Scholar 

  9. C. Guerra-Araiza, M.A. Cerbon, S. Morimoto, I. Camacho-Arroyo, Life Sci. 66, 1743–1752 (2000)

    Article  CAS  PubMed  Google Scholar 

  10. C. Leranth, N.J. Maclusky, T.J. Brown, E.C. Chen, D.E. Redmond Jr, F. Naftolin, Neuroendocrinology 55, 667–682 (1992)

    Article  CAS  PubMed  Google Scholar 

  11. I. Merchenthaler, M.L. Lane, S. Numan, T.L. Dellovade, J. Comp. Neurol. 473, 270–291 (2004)

    Article  CAS  PubMed  Google Scholar 

  12. H. Houben, C. Denef, Peptides 15, 547–582 (1994)

    Article  CAS  PubMed  Google Scholar 

  13. S. Reichlin, Ann. N. Y. Acad. Sci. 527, 431–449 (1988)

    Article  CAS  PubMed  Google Scholar 

  14. D.W. Hsu, P.N. Riskind, E.T. Hedley-Whyte, Am. J. Pathol. 135, 329–338 (1989)

    CAS  PubMed  Google Scholar 

  15. K. Köves, P.E. Gottschall, T.J. Görcs, J.G. Schammell, A. Arimura, Endocrinology 126, 1756–1763 (1990)

    Article  PubMed  Google Scholar 

  16. G.M. Nagy, J.J. Mulchahey, J.D. Neill, Endocrinology 122, 346–366 (1988)

    Google Scholar 

  17. J.W. Goldzieher, Perspect. Biol. Med. 36, 363–368 (1993)

    CAS  PubMed  Google Scholar 

  18. R. Kurzrok, J. Contracept. 2, 27–29 (1937)

    CAS  PubMed  Google Scholar 

  19. W.D. Müller-Janncke, Z. Gesamte, Inn. Med. 43, 420–422 (1988)

    Google Scholar 

  20. L.M. Senturk, C.T. Erel, Curr. Opin. Obstet. Gynecol. 20, 221–228 (2008)

    Article  PubMed  Google Scholar 

  21. J. Szekeres-Bartho, J. Balasch, Hum. Reprod. Update 14, 27–35 (2008)

    Article  PubMed  Google Scholar 

  22. H. Burger, Climacteric 1, 11–36 (2003)

    Google Scholar 

  23. L.J. Tivis, N.A. Ceballos, G. Chastain, R.D. Tivis, Neuropsychobiology 58, 104–110 (2008)

    Article  CAS  PubMed  Google Scholar 

  24. W.H. Utiah, D.F. Archer, G.A. Bachmann, C. Gallagher, F. Grodstein, J.R. Heiman, Menopause 15, 584–602 (2008)

    Article  Google Scholar 

  25. W.K. Oh, Clin. Prostate Cancer 1, 81–89 (2002)

    CAS  PubMed  Google Scholar 

  26. W.H. Rosténe, Prog. Neurobiol. 22, 103–129 (1984)

    Article  PubMed  Google Scholar 

  27. T.C. Hagen, I.M.A. Arnaout, W.J. Scherzer, D.R. Martinson, T.C. Garthwaite, Neuroendocrinology 43, 641–645 (1986)

    Article  CAS  PubMed  Google Scholar 

  28. J. Carretero, M. Angoso, M. Rubio, E.J. Blanco, E. Sierra, J.J. Herrero, E. Perez, D.J. Burks, Anat. Embryol. 211, 11–18 (2006)

    Article  CAS  PubMed  Google Scholar 

  29. K. Köves, I-Li Chen, T.J. Görcs, J. Scammell, A. Arimura, Endocrine 5, 219–223 (1996)

    Article  Google Scholar 

  30. R.A. Prysor-Jones, J.J. Silverlight, J.S. Jenkins, Neurosci. Lett. 80, 333–338 (1987)

    Article  CAS  PubMed  Google Scholar 

  31. J.A. Balsa, F. Sanchez-Franco, M.J. Lorenzo, F. Pazos, J.I. Lara, L. Cacicedo, Endocrinology 13, 508–513 (1996)

    Article  Google Scholar 

  32. O. Gómez, J.A. Balsa, Endocrinology 144, 4403–4409 (2003)

    Article  PubMed  Google Scholar 

  33. O. Gómez, J.A. Balsa, Neuroendocrinology 80, 324–331 (2004)

    Article  PubMed  Google Scholar 

  34. A. Heinzlmann, K. Köves, Endocrine 33, 342–348 (2008)

    Article  CAS  PubMed  Google Scholar 

  35. J.J. Calderon, T.G. Muldoon, V.B. Mahesh, Endocrinology 120, 2428–2435 (1987)

    Article  CAS  PubMed  Google Scholar 

  36. J.H. Casas, M.C. Chang, Biol. Reprod. 2, 315–325 (1970)

    Article  CAS  PubMed  Google Scholar 

  37. J.E. DeMaria, J.D. Livingston, M.E. Freeman, Brain Res. 879, 139–147 (2000)

    Article  CAS  PubMed  Google Scholar 

  38. A. Gulyás, T.J. Görcs, T. Freund, Neuroscience 37, 31–44 (1990)

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are greatly indebted to Ildikó Schay for her proofreading. This work was supported by the Department of Human Morphology and Developmental Biology and by the Hungarian Research Grant (OTKA-68170) to GMN.

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Correspondence to Katalin Köves.

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Heinzlmann, A., Köves, K. & Nagy, G.M. Effect of concomitant progesterone administration or the effect of removal of estrogen capsule on changes caused by long-term estrogen treatment in pituitary VIP immunoreactivities. Endocr 37, 396–402 (2010). https://doi.org/10.1007/s12020-010-9320-x

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  • DOI: https://doi.org/10.1007/s12020-010-9320-x

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