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Influence of Inoculum Source and Pre-incubation on Bio-Methane Potential of Chicken Manure and Corn Stover

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Abstract

In order to investigate the effects of inoculum source and pre-incubation on methane production performance of chicken manure (CM) and corn stover (CS), two sets of bio-methane potential tests using non- and pre-incubated inocula (digested sludge from a municipal wastewater treatment plant (DSMW) and digested sludge from a chicken manure treatment plant (DSCM)) were conducted at 37 °C. Modified Gompertz and first-order models were used to evaluate the kinetic parameters. The results revealed that DSMW was better than DSCM in digesting organic substrates (CM and CS), since the average ultimate methane yields were 351 mL g−1 volatile solid (VS)added for CM and 300 mL g−1 VSadded for CS when DSMW was used as inoculum, and 298 mL g−1 VSadded for CM and 218 mL g−1 VSadded for CS when DSCM was used as inoculum, respectively. Nevertheless, there was no significant difference (p > 0.05) in the ultimate methane yields between non- and pre-incubated inoculum for digesting CM and CS, regardless of the inoculum source. However, when evaluating the kinetic parameters of anaerobic digestion, the correlation coefficient, maximal methane production rate, and hydrolysis rate constant were slightly higher using pre-incubated inoculum as compared to non-incubated inoculum.

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Abbreviations

AD:

Anaerobic digestion

B d :

Biodegradability

BMP:

Bio-methane potential

CM:

Chicken manure

CS:

Chicken manure

DSCM:

Digested sludge from a chicken manure treatment plant

DSMW:

Digested sludge from a municipal wastewater treatment plant

N.S.:

No significant difference

S.:

Significant difference

SD:

Standard deviation

S/I ratio:

Substrate to inoculum ratio

TMY:

Theoretical methane yield

TS:

Total solids

TVFA:

Total volatile fatty acids

UMY:

Ultimate methane yield

VS:

Volatile solids

V.S.:

Very significant difference

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Acknowledgments

This research was supported by National Hi-tech R&D Program of China (863 Program, 2012AA101803) and National Natural Science Foundation of China (51108016). The authors gratefully acknowledge financial support from China Scholarship Council. We also appreciate assistance provided for proofreading and language correction by Mr. Steve Zicari, Department of Biological and Agricultural Engineering, and Ms. Hui Ean Teh, Food Science and Technology Department, University of California, Davis, USA.

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Authors and Affiliations

  1. Biomass Energy and Environmental Engineering Research Center, Beijing University of Chemical Technology, 503 Zonghe Building, 15 North 3rd Ring East Road, Beijing, 100029, People’s Republic of China

    Yeqing Li, Lu Feng, Ruihong Zhang, Yanfeng He, Xiaoying Liu, Xiao Xiao, Xinxin Ma, Chang Chen & Guangqing Liu

  2. Department of Biological and Agricultural Engineering, University of California, Davis, CA, 95616, USA

    Yeqing Li, Ruihong Zhang, Xiaoying Liu & Xinxin Ma

  3. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Chang Chen

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  1. Yeqing Li
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  2. Lu Feng
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  4. Yanfeng He
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  8. Chang Chen
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  9. Guangqing Liu
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Corresponding authors

Correspondence to Chang Chen or Guangqing Liu.

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Yeqing Li and Lu Feng contributed to this work equally.

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Li, Y., Feng, L., Zhang, R. et al. Influence of Inoculum Source and Pre-incubation on Bio-Methane Potential of Chicken Manure and Corn Stover. Appl Biochem Biotechnol 171, 117–127 (2013). https://doi.org/10.1007/s12010-013-0335-7

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