Summary
When fetal rat long bones are incubated in the presence of 10−8 M 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], steady-state parathyroid hormone-related peptide (PTHrP) mRNA levels are decreased. This decrease is temporary: it is observed as soon as after 3 h of exposure and reaches a nadir after 6 h. At that time, PTHrP mRNA levels are significantly lower in the experimental than in the control bones. However the inhibitory effect vanishes after 24 h, despite continuous exposure to 1,25(OH)2D3 for even 48 h. This is the first report showing that PTHrP mRNA expression can be regulated in rat fetal long bones in vitro by 1,25(OH)2D3.
Similar content being viewed by others
References
Amizuka, N.; Henderson, J. E.; Hoshi, K.; Warshawsky, H.; Ozawa, H.; Goltzman, D.; Karaplis, A. C. Programmed cell death of chondrocytes and aberrant chondrogenesis in mice homozygous for parathyroid hormone-related peptide gene deletion. Endocrinology 137:5055–5067; 1996.
Endo, K.; Ichikawa, F.; Uchiyama, Y.; Katsumata, K.; Ohkawa, H.; Kumaki, K.; Ogata, E.; Ikeda, K. Evidence for the uptake of a vitamin D analogue (OCT) by a human carcinoma and its effect of suppressing the transcription of parathyroid hormone-related peptide gene in vivo. J. Biol. Chem. 269:32693–32699; 1994.
Falzon, M. The noncalcemic vitamin D analogues EB1089 and 22-oxacalcitriol interact with the vitamin D receptor and suppress parathyroid hormone-related peptide gene expression. Mol. Cell. Endocrinol. 127:99–108; 1997.
Ikeda, K.; Lu, C.; Weir, E. C.; Mangin, M.; Broadus, A. E. Transcriptional regulation of the parathyroid hormone-related peptide gene by glucocorticoids and vitamin D in human C-cell line. J. Biol. Chem. 264:15743–15746; 1989.
Inoue, D.; Matsumoto, T.; Ogata, E.; Ikeda, K. 22-Oxacalcitriol, a non-calcemic analogue of calcitriol, suppresses both cell proliferation and parathyroid hormone-related peptide gene expression in human T cell lymphotrophic virus, type I-infected T cells. J. Biol. Chem. 268:16730–16736; 1993.
Karmali, R.; Bergmann, P.; Schifmann, S. N.; Vanderwinden, J. M.; Hendy, G. N.; Nijs-DeWolf, N.; Corvilain, J.; Vanderhaeghen, J. J. Expression of parathyroid hormone-related peptide in rat fetal bones. In: Calcium regulating hormones and bone metabolism: basic and clinical aspects. Amsterdam: Elsevier Sciences Publishers B.V.; 1992:67–68.
Karmali, R.; Schiffmann, S. N.; Vanderwinden, J. M.; Hendy, G. N.; Nijs-DeWolf, N.; Corvilain, J.; Bergmann, P.; Vanderhaeghen, J. J. Expression of mRNA of parathyroid hormone-related peptide in fetal bones of the rat. Cell Tissue Res. 270:597–600; 1992.
Kartsogiannis, V.; Moseley, J.; McKelvie, B.; Chou, S. T.; Hards, D. K.; Ng, K. W.; Martin, T. J.; Zhou, H. Temporal expression of PTHrP during endochondral bone formation in mouse and intramembranous bone formation in an in vivo rabbit model. Bone 21:385–392; 1997.
Kremer, R.; Karaplis, A. C.; Henderson, J.; Gulliver, W.; Banville, D.; Hendy, G. N.; Goltzman, D. Regulation of parathyroid hormone-like peptide in cultured normal human keratinocytes. J. Clin. Invest. 87:884–893; 1991.
Kremer, R.; Sebag, M.; Champigny, C.; Meerovitch, K.; Hendy, G. N.; White, J.; Goltzman, D. Identification and characterization of 1,25-dihydroxyvitamin D3-responsive repressor sequences in the rat parathyroid hormone-related peptide-gene. J. Biol. Chem. 271:16310–16316; 1996.
Lee, K.; Deeds, J. D.; Segre, G. V. Expression of parathyroid hormone-related peptide and its receptor messenger ribonucleic acids during fetal development of rat. Endocrinology 136:453–463; 1995.
Lee, K.; Lanske, B.; Karaplis, A. C.; Deeds, J. D.; Kohno, H.; Nissenson, R. A.; Kronenberg, H. M.; Segre, G. V. Parathyroid hormone-related peptide delays terminal differentiation of chondrocytes during endochondral bone development. Endocrinology 137:5109–5118; 1996.
Liu, B.; Goltzman, D.; Rabbani, S. A. Regulation of parathyroid hormone-related peptide production in vitro by the rat hypercalcemic Leydig cell tumor H-500. Endocrinology 132:1658–1664; 1993.
Nijs-DeWolf, N.; Pepersack, T.; Corvilain, J.; Karmali, R.; Bergmann, P. An adenylate cyclase stimulating activity immunologically similar to parathyroid hormone-related peptide can be extracted from rat long bones. J. Bone Min. Res. 6:921–927; 1991.
Raisz, L. G.; Nieman, I. Effects of phosphate, calcium and magnesium on bone resorption and hormonal responses in tissue culture. Endocrinology 85:446–452; 1969.
Weir, E. C.; Philbrick, W. M.; Amling, M.; Neff, L. A.; Baron, R.; Broadus, A. E. Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation. Proc. Natl. Acad. Sci. USA 93:10240–10245; 1996.
Xie, L. Y.; Leung, A.; Segre, G. V.; Yamamoto, I.; Abou-Samra, A. B. Downregulation of the PTH/PTHrP receptor by vitamin D3 in the osteoblast-like ROS 17/2.8 cells. Am. J. Physiol. 270:E654-E660; 1996.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Karmali, R., Nijs-DeWolf, N., Beyer, I. et al. 1,25-Dihydroxyvitamin D3 inhibits parathyroid hormone-related peptide mRNA expression in fetal rat long bones in culture. In Vitro Cell.Dev.Biol.-Animal 35, 296–298 (1999). https://doi.org/10.1007/s11626-999-0074-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11626-999-0074-9