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The molecular signature and spermatogenesis potential of newborn chicken spermatogonial stem cells in vitro

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Abstract

Although chicken spermatogonial stem cells (SSCs) have received considerable attention in recent years, only a few studies so far have focused on their derivation and characterization in vitro. Identification of specific molecular biomarkers and differentiation capacity of chicken SSCs would not only help us to understand cell and molecular biology of these cells, but also can contribute to their applications in biotechnology. In this regard, we found that colony-forming cells (SSCs) in newborn chicken testicular cell cultures were positive for alkaline phosphatase activity and also expressed specific markers including DAZL, STRA-8, CVH, PLZF, SPRY-1, GFRα1, GDNF, POU5F1, NANOG, GPR125, THY-1, c-KIT, and BCL6B, at mRNA level. Moreover, these cells expressed POU5F1 and GPR125 proteins as reliable intracellular and cell surface markers, respectively; whereas they were negative for SSEA-1. Furthermore, we showed that newborn chicken colony-forming cells had spermatogenesis potential and thus could be produced sperm-like cells in a three-dimensional matrix in vitro. In conclusion, this study reports novel insights into the molecular signature of newborn chicken SSCs in comparison with mammalian SSCs and for the first time we report a successful protocol for in vitro spermatogenesis and thus production of sperm-like cells from newborn chicken testicular cell cultures.

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Acknowledgments

The authors would like to thank Morghak Co. for generously providing the chickens. This work was supported by grants from Ferdowsi University of Mashhad (No. 20880 and No. 100313) which are highly appreciated. We are also thankful to Mrs. Fatemeh B. Rassouli and Mr. Nakhaei for their great assistance.

Conflict of interest

The authors declare no conflicts of interest.

Author information

Author notes

  1. Sajjad Sisakhtnezhad

    Present address: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran

Authors and Affiliations

  1. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Sajjad Sisakhtnezhad, Ahmad Reza Bahrami, Maryam M. Matin, Moein Farshchian & Mahtab Dastpak

  2. Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, P.O. Box: 9177948974, Iran

    Sajjad Sisakhtnezhad, Ahmad Reza Bahrami, Maryam M. Matin, Hesam Dehghani, Moein Farshchian & Mahtab Dastpak

  3. Department of Basic Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

    Hesam Dehghani

  4. Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran

    Madjid Momeni-Moghaddam

  5. Department of Biology, Faculty of Basic Science, Gonbad Kavous University, Gonbad Kavous, Iran

    Sohrab Boozarpour

Authors
  1. Sajjad Sisakhtnezhad
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  2. Ahmad Reza Bahrami
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  4. Hesam Dehghani
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  5. Madjid Momeni-Moghaddam
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  6. Sohrab Boozarpour
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  7. Moein Farshchian
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  8. Mahtab Dastpak
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Corresponding author

Correspondence to Ahmad Reza Bahrami.

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Editor: T. Okamoto

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Sisakhtnezhad, S., Bahrami, A.R., Matin, M.M. et al. The molecular signature and spermatogenesis potential of newborn chicken spermatogonial stem cells in vitro. In Vitro Cell.Dev.Biol.-Animal 51, 415–425 (2015). https://doi.org/10.1007/s11626-014-9843-1

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  • DOI: https://doi.org/10.1007/s11626-014-9843-1

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