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Pileospora piceae gen. et sp. nov. (Septorioideaceae, Botryosphaeriales) from Picea rubens

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Abstract

During a survey of conifer-associated fungi from the Acadian forest in Eastern Canada, an unidentified endophyte species was isolated from surface-sterilized red spruce (Picea rubens) needles. Conidiomata developed in vitro and produced dimorphic conidia, which provided morphological evidence of its position within Botryosphaeriales. Corresponding conidiomata were also observed on a dead red spruce twig. Phylogenetic analyses using nuc internal transcribed spacer rDNA (ITS) and partial 28S nuc rDNA (LSU) sequences confirmed its relationship within Botryosphaeriales, in a polytomy containing Saccharataceae and Septorioideaceae. Based on morphological and phylogenetic evidence, we describe a novel monotypic genus and species as Pileospora piceae, tentatively placed within Septorioideaceae. ITS similarity shows P. piceae was previously detected in an unrelated study as an unidentified fungal endophyte of black spruce (Picea mariana) from Eastern Quebec.

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Acknowledgements

We are grateful to the MMTL group of ORDC-AAFC, Ottawa, for processing DNA sequencing. Dr. J.D. Miller and Dr. M.E. Smith of Carleton University provided valuable support in the planning and implementation of this work.

Funding

This study was supported by the Natural Sciences and Engineering Research Council of Canada PGSD2-459312-2014 to J.B. Tanney and the NSERC CRDPJ 421782-11 to J.D. Miller, K.A. Seifert, and D.W. Malloch.

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Authors and Affiliations

  1. Biodiversity (Mycology & Botany), Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada

    Joey B. Tanney & Keith A. Seifert

  2. Department of Biology, Carleton University, 209 Nesbitt Biology Building, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada

    Joey B. Tanney

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  1. Joey B. Tanney
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Correspondence to Joey B. Tanney.

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Section Editor: Hans-Josef Schroers and Marc Stadler

This article is part of the “Special Issue on hyphomycete taxonomy and diversity in honour of Walter Gams who passed away in April 2017”.

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Tanney, J.B., Seifert, K.A. Pileospora piceae gen. et sp. nov. (Septorioideaceae, Botryosphaeriales) from Picea rubens. Mycol Progress 18, 163–174 (2019). https://doi.org/10.1007/s11557-018-1423-4

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