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Expression of the Mannose Receptor CD206 in HIV and SIV Encephalitis: A Phenotypic Switch of Brain Perivascular Macrophages with Virus Infection

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Abstract

We examined the expression of the mannose receptor CD206 by perivascular macrophages (PVM) in normal human and monkey brains and in brains of HIV-infected humans and of monkeys infected with simian immunodeficiency virus (SIV). Depletion of brain PVM in SIV-infected monkeys by intrathecal injection of liposome-encapsulated bisphosphonates eliminated CD206-expressing cells in the brain, confirming their perivascular location and phagocytic capacity. In vivo labeling with bromodeoxyuridine in normal uninfected and SIV-infected macaques in combination with CD206 immunostaining revealed a CD206+-to-CD206– shift within pre-existing PVM during SIV brain infection and neuroinflammation. These findings identify CD206 as a unique marker of human and macaque PVM, and underscore the utility of this marker in studying the origin, turnover and functions of these cells in AIDS.

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Acknowledgments

This study was supported by grant funding from Virginia’s Commonwealth Health Research Board to W.K.K (#11-09) and in part by Public Health Service grants R21AI091501, R21AI110163 and R01AI097059 to M.J.K. We thank Dr. Susan Morgello, of the Manhattan HIV Brain Bank (R24MH59724), for facilitating access to HIVE brain autopsy samples.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 W Olney Road, Lewis Hall 3174, Norfolk, VA 23507, USA

    Gerard E. Holder, Christopher M. McGary, Edward M. Johnson & Woong-Ki Kim

  2. Department of Chemical and Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA

    Rubo Zheng & Vijay T. John

  3. Division of Immunology, Tulane National Primate Research Center, Covington, LA 70433, USA

    Chie Sugimoto & Marcelo J. Kuroda

Authors
  1. Gerard E. Holder
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  2. Christopher M. McGary
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  3. Edward M. Johnson
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  5. Vijay T. John
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  6. Chie Sugimoto
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  7. Marcelo J. Kuroda
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  8. Woong-Ki Kim
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Correspondence to Woong-Ki Kim.

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Gerard E. Holder and Christopher M. McGary contributed equally to the work.

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Holder, G.E., McGary, C.M., Johnson, E.M. et al. Expression of the Mannose Receptor CD206 in HIV and SIV Encephalitis: A Phenotypic Switch of Brain Perivascular Macrophages with Virus Infection. J Neuroimmune Pharmacol 9, 716–726 (2014). https://doi.org/10.1007/s11481-014-9564-y

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  • DOI: https://doi.org/10.1007/s11481-014-9564-y

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