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Generation of the genetic mutant population for the screening and characterization of the mutants in response to drought in maize

  • Article
  • Physiology
  • Published:
Chinese Science Bulletin

Abstract

The autonomous Mutator (Mu) transposon in maize (Zea mays L.) lines 115F, 330I, and 715D were crossed with inbred lines B73, Mo17, 97108, and H9-21, M1 were self-pollinated to establish the Mu insertion-mutagenized M2 seeds pool and The M2 plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the field. Next we statistically analyzed these candidate mutants. Under drought stress, we screened seedlings at 3-leaf stage by using the infrared thermography, and successfully got 108 drought-insensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines, which temperature were significant different with others. These candidate mutants were primarily analyzed by infrared thermography, chlorophyll fluorescence, leaf water losing, photosynthetic characteristics, content of soluble sugars, soluble protein, and proline assay. The selected mutants were cloned by MuTAIL–PCR methods. Here we provide the better genetic materials for research on maize breeding for drought tolerance.

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Acknowledgments

This work was supported by the National Key Basic Research and Development Program of China (2012CB114301).

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Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Kaifeng, 475004, China

    Zhiyong Gao, Daojie Wang & Chunpeng Song

  2. Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng, 475004, China

    Hao Liu, Hongliang Wang, Ning Li, Yuwei Song, Yuchen Miao & Chunpeng Song

Authors
  1. Zhiyong Gao
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  2. Hao Liu
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  3. Hongliang Wang
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  4. Ning Li
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  5. Daojie Wang
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  6. Yuwei Song
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  7. Yuchen Miao
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  8. Chunpeng Song
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Correspondence to Chunpeng Song.

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Gao, Z., Liu, H., Wang, H. et al. Generation of the genetic mutant population for the screening and characterization of the mutants in response to drought in maize. Chin. Sci. Bull. 59, 766–775 (2014). https://doi.org/10.1007/s11434-013-0031-6

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  • DOI: https://doi.org/10.1007/s11434-013-0031-6

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