Abstract
The scant hair mutant mouse (locus symbol: snthr 1Bao) is a recessive mutation that originated in an ethylnitrosourea chemical carcinogenesis study using the DBA/2J inbred strain. The gene responsible for the mutation was previously determined to be phospholipase C, delta 1 (Plcd1; mutant allele symbol Plcd1 snthr1Bao). To map the modifiers of Plcd1, an intercross (DBA/2J-snthr 1Bao/snthr 1Bao × C57BL/6J+/+) was conducted. The F2 mutant progeny exhibited a variety of alopecia phenotypes; all F2 mutants (n=507) were classified into 3 groups (mild, moderate, and severe alopecia) and genotyped based on 96 microsatellites. A major QTL was identified on mouse chromosome (mChr) 15 at 12 cM with an LOD score greater than 7 (P < 0.0001). Three minor QTLs were detected on mChr 2, 5, and 7 at 40, 84 and 48 cM, respectively. The QTLs on mChr 7 and 15 were associated with minor alopecia while the QTLs on mChr 2 and 5 were associated with moderate to severe alopecia. No antagonistic or synergistic effects among or between the 4 QTLs were found. Integrating the functions of the 4 potential regulatory QTLs and mutant Plcd1 snthr1Bao, we found that these QTLs might contribute to variations of scant hair severity by altering the Ca2+ signal pathways in mouse skin.
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Wu, B., Zeng, Y., Mao, H. et al. Mapping of genetic modifiers of Plcd1 in scant hair mice (snthr 1Bao). Chin. Sci. Bull. 55, 4026–4031 (2010). https://doi.org/10.1007/s11434-010-4075-6
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DOI: https://doi.org/10.1007/s11434-010-4075-6