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Preparing a re-sequencing DNA library of 2 cancer candidate genes using the ligation-by-amplification protocol by two PCR reactions

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Abstract

To meet the needs of large-scale genomic/genetic studies, the next-generation massively parallelized sequencing technologies provide high throughput, low cost and low labor-intensive sequencing service, with subsequent bioinformatic software and laboratory methods developed to expand their applications in various types of research. PCR-based genomic/genetic studies, which have significant usage in association studies like cancer research, haven’t benefited much from those next-generation sequencing technologies, because the shortgun re-sequencing strategy used by such sequencing machines as the Illumina/Solexa Genome Analyzer may not be applied to direct re-sequencing of short-length target regions like those in PCR-based genomic/genetic studies. Although several methods have been proposed to solve this problem, including microarray-based genomic selections and selector-based technologies, they require advanced equipment and procedures which limit their applications in many laboratories. By contrast, we overcame such potential drawbacks by utilizing a ligation by amplification (LBA) protocol, a method using a pair of Universal Adapters to randomly ligate target regions in a two-step-PCR procedure, whose Long LBA products were easily fragmented and sequenced on the next-generation sequencing machine. In this concept-proven study, we chose the consensus coding sequences of two human cancer genes: BRCA1 and BRCA2 as target regions, specifically designed LBA primer pairs to amplify and randomly ligate them. 70 target sequences were successfully amplified and ligated into Long LBA products, which were then fragmented to construct DNA libraries for sequencing on both a conventional Sanger sequencer ABI 3730xl DNA Analyzer and the next-generation ‘synthesis by sequencing technology’ Illumina/Solexa Genome Analyzer. Bioinformatic analysis demonstrated the utility and efficiency (including the coverage and depth of each target sequence and the SNPs detection effectiveness) of using the LBA protocol in facilitating PCR-based re-sequencing and genetic-variant-detection studies on the next-generation sequencing machine, raising the prospect of various PCR-based genomic/genetic studies using this strategy.

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Authors and Affiliations

  1. Graduate School of Chinese Academy of Sciences, Beijing, 100049, China

    YeYang Su, Lin Lin, Geng Tian & XinPeng Qi

  2. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China

    YeYang Su, Lin Lin, Geng Tian, Chen Chen, Tao Liu, XinPeng Qi, XiuQing Zhang & HuanMing Yang

  3. Beijing Genomics Institute, Beijing, 101300, China

    YeYang Su, Lin Lin, Geng Tian, Chen Chen, Tao Liu, Xingya Xu, XinPeng Qi, XiuQing Zhang & HuanMing Yang

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  1. YeYang Su
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  2. Lin Lin
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  3. Geng Tian
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  4. Chen Chen
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  5. Tao Liu
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  6. Xingya Xu
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  7. XinPeng Qi
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  8. XiuQing Zhang
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  9. HuanMing Yang
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Corresponding authors

Correspondence to XiuQing Zhang or HuanMing Yang.

Additional information

National High-Tech Research & Development Program of China (Grant No.2006 AA02A301)

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Su, Y., Lin, L., Tian, G. et al. Preparing a re-sequencing DNA library of 2 cancer candidate genes using the ligation-by-amplification protocol by two PCR reactions. SCI CHINA SER C 52, 483–491 (2009). https://doi.org/10.1007/s11427-009-0066-8

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  • DOI: https://doi.org/10.1007/s11427-009-0066-8

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