Abstract
This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chromatographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.
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Support from the Major State Basie Research Development Program (Grant No. 2007CB914100), the National Natural Science Foundation of China (Grant Nos. 20875016 & 20735005), and Shanghai Projects (Grant Nos. 08DZ2293601 & B109).
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Zhang, X., Liu, B., Zhang, L. et al. Recent advances in proteolysis and peptide/protein separation by chromatographic strategies. Sci. China Chem. 53, 685–694 (2010). https://doi.org/10.1007/s11426-010-0135-7
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DOI: https://doi.org/10.1007/s11426-010-0135-7