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Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

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Abstract

Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

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Authors and Affiliations

  1. Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China

    Hao Yue, Wan Chan, Lin Guo, Ning Liu & ZongWei Cai

  2. Changchun University of Chinese Medicine, Changchun, 130117, China

    Hao Yue & Ning Liu

  3. Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, 350001, China

    KongJie Yu

  4. Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    HuWei Liu

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  1. Hao Yue
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  2. Wan Chan
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  3. KongJie Yu
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  4. Lin Guo
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Correspondence to ZongWei Cai.

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Supported by the Research Grant Council, University Grants Committee of Hong Kong (Grant No. HKBU2459/06M), and the Health and Health Services Research Fund of Hong Kong (Grant No. 05060141)

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Yue, H., Chan, W., Yu, K. et al. Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid. Sci. China Ser. B-Chem. 52, 1576–1582 (2009). https://doi.org/10.1007/s11426-009-0233-6

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