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Fluorescence dynamics of interactions between polyamide PyPyPyβDp and DNA

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Abstract

The photophysical properties of the polyamide PyPyPyβDp (PPP) were investigated by means of steady-state absorption and fluorescence spectroscopies, as well as time-resolved fluorescence spectroscopy. It was found that the excited-state properties of PPP are very sensitive to solvents. In TKMC buffer PPP exhibited weak fluorescence with a decay time constant of 16 ps, while with the decrease of the solvent polarity PPP showed the blue-shifted peak position, increased intensity and lengthened life-time for its fluorescence behavior. In the presence of calf thymus DNA, it was observed that the fluorescence intensity was enhanced and the fluorescence lifetime increased from 16 to 32 ps for PPP, which verified that PPP bound into the minor groove of DNA duplex.

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Authors and Affiliations

  1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, China

    Zhang Huijuan, Wu Yishi, Ai Xicheng & Zhang Jianping

  2. School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, China

    Zhang Huijuan

  3. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100080, China

    Wang Jin & Yuan Gu

  4. Center for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100080, China

    Wang Li

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  1. Zhang Huijuan
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  2. Wang Jin
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  3. Wu Yishi
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  4. Yuan Gu
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  5. Ai Xicheng
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  6. Wang Li
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  7. Zhang Jianping
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Correspondence to Ai Xicheng or Zhang Jianping.

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Zhang, H., Wang, J., Wu, Y. et al. Fluorescence dynamics of interactions between polyamide PyPyPyβDp and DNA. SCI CHINA SER B 49, 75–80 (2006). https://doi.org/10.1007/s11426-005-0025-6

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  • DOI: https://doi.org/10.1007/s11426-005-0025-6

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