Abstract
Soil salinity is a major constraint that limits legume productivity. Pigeonpea is a salt sensitive crop. Seed gamma irradiation at a very low dose (2.5 Gy) is known to enhance seedling establishment, plant growth and yield of cereals and other crops. The present study conducted using two genetically diverse varieties of pigeonpea viz., Pusa-991 and Pusa-992 aimed at establishing the role of pre-sowing seed gamma irradiation at 0, 0.0025, 0.005, 0.01, 0.02, 0.05 and 0.1 kGy on plant growth, seed yield and seed quality under salt stress at 0, 80 and 100 mM NaCl (soil solution EC equivalent 1.92, 5.86 and 8.02 dS/m, respectively) imposed right from the beginning of the experiment. Changes in carbon flow dynamics between shoot and root and concentration of osmolyte, glycine betaine, plant uptake and shoot and root partitioning of Na+ and K+ and activity of protein degrading enzyme protease were measured under the combined effect of gamma irradiation and salt stress. Positive affect of pre-sowing exposure of seed to low dose of gamma irradiation (<0.01 kGy) under salt stress was evident in pigeonpea. Pigeonpea variety, Pusa-992 showed a better salt tolerance response than Pusa-991 and that the radiated plants performed better than the unirradiated plants even at increasing salinity level. Seed yield and seed protein and iron content were also positively affected by the low dose gamma irradiation under NaCl stress. Multiple factors interacted to determine physiological salt tolerance response of pigeonpea varieties. Gamma irradiation caused a favourable alteration in the source-sink (shoot-root) partitioning of recently fixed carbon (14C) under salt stress in pigeonpea. Gamma irradiation of seeds prior to sowing enhanced glycine betaine content and reduced protease activity at 60-day stage under various salt stress regimes. Lower partitioning of Na+and relatively higher accumulation of K+ under irradiation treatment was the other important determinants that differentiated between salt-tolerant and salt-susceptible variety of pigeonpea. The study provides evidence and physiological basis for exploring exploitation of pre-sowing exposure of seeds with low-dose gamma ray for enhancing the salt tolerance response of crop plants.
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Abdel Haleem M, Mohammed A, Mohammed HI, Zaki LM, Mogazy AM (2012) Pre-exposure to gamma ray eliminates the harmful effect of salinity on cowpea plants. J Stress Physiol Biochem 8:199–217
Ahuja S, Malhotra PK, Bhatia PK and Parsad R (2008) Statistical package for agricultural research (SPAR 2.0). J Ind. Soc. Agril. Statist. 62(1): 65-74
Ahuja S, Kumar M, Kumar P, Gupta VK, Singhal RK, Yadav A, Singh B (2014) Metabolic and biochemical changes caused by gamma irradiation in plants. J Radioanal Nucl Chem 299:2969–2929
Amuthavalli P, Sivasankaramoorthy S (2012) Effect of salt stress on the growth and photosynthetic pigments of pigeonpea. J App Pharrm Sci 2:131–133
Arakawa T, Timasheff SN (1985) The stabilization of proteins by osmolytes. Biophy J 47:411–414
Ashraf MY, Ashraf M, Mahmood K, Akhter J, Hussain F, Arshad M (2010) Phytoremediation of saline soils for sustainable agricultural productivity. In: Ashraf M, Oztruck M, Ahmad MSA (eds) Plant adaptation and phytoremediation. Springer Science+Business Media B.V, the Netherlands, pp 335–356
Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16
Barunawati N, Giehl RFH, Bauer B, von Wirén N (2013) The influence of inorganic nitrogen fertilizer forms on micronutrient retranslocation and accumulation in grains of winter wheat. Front Plant Sci 4:320
Bhargava BS, Raghupathi HB (1993) Analysis of plant materials for macro and micronutrients. In: Tandon HLS (ed) Methods of analysis of soils, plants, water and fertilizers. Fertilization Department Consultant Organization, New Delhi, pp 49–82
Blum A (1985) Breeding crop varieties for stress environments. CRC Critical Rev Plant Sci 2:219
Borzouei A, Kafi M, Khazaei H, Naseriyan B, Majdabadi A (2010) Effect of gamma radiation on germination and physiological aspects of wheat (Triticum aestivum L.) seedlings. Pak J Bot 42(4):2281–2290
Bulut, F and Akıncı, Ş (2010) The effect of salinity on growth and nutrient composition in broad bean (Vicia faba L.) seedlings. Fresenius Environmental Bulletin by PSP Volume 19 – No 12. 2010.
Celik O, Atak C (2012) The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties. Turk J Biol 36:339–356
Demiral P, Turkan I (2004) Does exogenous glycine betaine affect antioxidative system of rice seedlings under NaCl treatment. J of Plant Physiol 161:1089–1100
Desai SA, Rao S (2014) Effect of gamma radiation on germination and physiological aspects of pigeonpea (Cajanus cajan L.) seedlings. Int J Res App Nat Soc Sc 2(6):47–52
Diaz-Zorita M, Fernandez-Cainigia MV, Grosso GA (2001) Application of foliar fertilizers containing glycine betaine improve wheat yields. J Agron Crop Sci 186:209–215
Dilkes NB, Jones DL, Farrar J (2004) Temporal dynamics of carbon partitioning and rhizodeposition in wheat. Plant Physiol 134:706–715
Grieve CM and Grattan SR (1983) Rapid assay for determination of water soluble quaternary ammonium compounts. Plant Soil. 70: 303-307
Kim JS, Lee EK, Back MH, Kim DH, Lee YB (2000) Influence of low dose γ radiation on the physiology of germinative seed of vegetable crops. Kor J Environ Agric 19:58–61
Krishnan V, Singh A, Vinutha T, Singh B, Dahuja A, Rai R, Sachdev A (2015) Low gamma irradiation effects on protein profile, solubility, oxidation, scavenger ability and bioavailability of essential minerals in black and yellow Indian soybean (Glycine max L.) varieties. J Rad Nuc Chem 10:4193–4193
Kumar P, Sharma V, Raje RS, Singh B (2016) Low dose gamma irradiation induces water activity, leaf K+/Na+, glycine betaine, antioxidant enzyme activity and reduces lipid peroxidation and protease activity to enhance salt tolerance in pigeonpea [Cajanus cajan (L.) Millsp]. J Radl Nuc Chem 308:965–980
Lowry OH, Resebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275
Makela P, Karkkainen J, Somersalo S (2000) Effect of glycine betaine on chloroplast ultrastructure, chlorophyll and protein content and RUBPCO activities in tomato grown under drought or salinity. Biol Plant 43:471–475
Manchanda G, Garg N (2008) Salinity and its effects on the functional biology of legumes. Acta Physiol Plant 30(5):595–618
Marschner H (1995) Mineral nutrition of higher plants. Academic Press New York, Second Edition
Munns R (2002) Comparative physiology of salt and water stress. Plant cell Environ. 25:239-250
Nieri B, Canino S, Versace R, Alpi A (1998) Purification and characterization of an endoprotease from alfalfa senescent leaves. Phytochem 49(3):643–649
Pandey R, Krishnapriya V, Kishora N, Singh SB, Singh B (2014) Shoot labelling with 14CO2: a technique for assessing total root carbon exudation under phosphorus stress. Ind J Plant Physiol 18:250–262
Parida AK, Das AB, Mittra B, Mohanty P (2004) Salt-stress induced alterations in protein profile and protease activity in the mangrove Bruguiera parviflora. Z Naturforsch C 59:408–414
Qadar A (1995) Potassium and sodium contents of shoot and laminae of rice cultivars and their sodicity tolerance. J Plant Nutr 18:2281–2290
Qi W, Zhang L, Xu H, Wang L, Jiao Z (2014) Physiological and molecular characterization of the enhanced salt tolerance induced by low-dose gamma irradiation in Arabidopsis seedlings. Biochem Biophys Res Commun 25(2):1010–1015
Rejili M, Telahigue D, Lachiheb B., Mrabet A, Ferchichi A (2008) Impact of gamma radiation and salinity on growth and K+/Na+ balance in two populations of medicago sativa (L.) cultivar Gabes. Progress Natural Sci. 18:1095-1105
Rhodes D, Rich PJ, Brunk DG, Ju GC, Rhodes JC, Pauly MH, Hansan LA (1989) Development of two isogenic sweet corn hybrids differing in glycine betaine content. Plant Physiol 91:1112–1121
Sahid MA, Ashraf MY, Pervez MA, Ahmed R, Balal RM, Sanchez FG (2013) Impact of salt stress on concentration of sodium+, Cl+ and organic solutes concentration in pea cultivars. Pak J Bot 45:755–761
Sairam RK, Tyagi A (2004) Physiology and molecular biology of salinity stress tolerance in plants. Cur Sci 86(3):407–421
Singh B, Datta PS (2010) Gamma irradiation to improve plant vigour, grain development, and yield attributes of wheat. Rad Phys Chem 79:139–143
Singh B, Singh BK, Yadav SS, Kuar J, Usha K (2005) Effects of salt stress on growth, nodulation, and nitrogen and carbon fixation of ten chickpea genotypes. Aust J Agri Res 56(5):491–495
Singh B, Ahuja S, Singhal RK, Venu BP (2014) Radiosensitivity studies and radiostability of ribulose-1,5 bis-carboxylase and gas exchange characteristics in wheat, garden pea, field pea, spinach, and okra. Water Air and Soil Pollution 10(13):1815–1817
Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. Lowa State University Press, Ames, p 480
Stewart GR, Lee JA (1974) Role of proline accumulation in halophytes. Planta 120:279–289
Tewari K, Kumari S, Vinutha T, Singh B, Dahuja A (2014) Gamma irradiation induces reduction in the off flavour generation in soybean through enhancement of its antioxidant potential. J Rad Nuc Chem. 302:3803–3809
Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC Gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301
Waheed A., Hafiz IA., Qadir G., Murtaza G, Mahmood T and Ashraf M (2006) Effect of salinity on germination, growth, yield, ionic balance and solute composition of pigeonpea (Cajanus Cajan (L.) Millsp.) Pak J Bot. 38 : 1103
Wi SG, Chung BY, Kim JS (2007) Effects of gamma irradiation on morphological changes and biological responses in plants. Micron 38:553–564
Wi SG, Chung BY, Kim JH, Baek MH, Yang DH, Lee JH, Kim JS (2005) Ultrastructural changes of cell organelles in Arabidopsis stem after gamma irradiation. J Plant Biol 48(2):195–200
Zhu JK (2002) Plant salt tolerance. Trends Plant Sci 61:66–71
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The scholarship provided to the first author by the Indian Agricultural Research Institute for doctorate degree is gratefully acknowledged.
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Kumar, P., Sharma, V., Atmaram, C.K. et al. Regulated partitioning of fixed carbon (14C), sodium (Na+), potassium (K+) and glycine betaine determined salinity stress tolerance of gamma irradiated pigeonpea [Cajanus cajan (L.) Millsp]. Environ Sci Pollut Res 24, 7285–7297 (2017). https://doi.org/10.1007/s11356-017-8406-x
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DOI: https://doi.org/10.1007/s11356-017-8406-x