Abstract
Pichia pastoris: (reclassified as Komagataella phaffii), a methylotrophic yeast strain has been widely used for heterologous protein production because of its unique advantages, such as readily achievable high-density fermentation, tractable genetic modifications and typical eukaryotic post-translational modifications. More recently, P. pastoris as a metabolic pathway engineering platform has also gained much attention. In this mini-review, we addressed recent advances of molecular toolboxes, including synthetic promoters, signal peptides, and genome engineering tools that established for P. pastoris. Furthermore, the applications of P. pastoris towards synthetic biology were also discussed and prospected especially in the context of genome-scale metabolic pathway analysis.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (31670092), the Natural Science Foundation of Jiangsu Province (BK20141107), a grant from the Key Technologies R&D Program of Jiangsu Province, China (BE2014607); Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R26).
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Kang, Z., Huang, H., Zhang, Y. et al. Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications. World J Microbiol Biotechnol 33, 19 (2017). https://doi.org/10.1007/s11274-016-2185-2
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DOI: https://doi.org/10.1007/s11274-016-2185-2