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Decolorization of Victoria blue by the white rot fungus, Phanerochaete chrysosporium

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Abstract

Synthetic textile dyes are among the most dangerous chemical pollutants released in industrial wastewater streams. Recognizing the importance of reducing the environmental impact of these dyes, the ability of the white rot fungus Phanerochaete chrysosporium to decolorize various textile dyes was investigated. This fungus decolorized 6 of the 14 structurally diverse dyes with varying efficiency (between 14% and 52%). There was no discernable pattern of decolorization even among dyes of the same chemical class, suggesting that attack on the dyes is relatively non-specific. Among the three dyes which showed >40% decolorization, Victoria Blue B (VB) was chosen for further analysis because the ability of the fungus to decolorize VB was nearly independent over a relatively broad concentration range. Blocking lignin peroxidase (LiP) and manganese peroxidase (MnP) production by the fungus did not substantially affect VB decolorization. Inhibition of laccase production by adding various inhibitors to shaken cultures reduced VB decolorization significantly suggesting a role for laccase in VB decolorization. When sodium azide and aminotriazole were used to inhibit endogenous catalase and cytochrome P-450 oxygenase activities, there was 100% and 70% reduction in VB decolorization, respectively. Adding benzoate to trap hydrogen peroxide-derived hydroxyl radicals resulted in 50% decolorization of VB. Boiling the extracellular fluid (ECF) for 30 min resulted in approximately 50% reduction in VB decolorization. Collectively, these data suggest that laccase, and/or oxygenase/oxidase and a heat-stable non-enzymatic factor, but not Lip and MnP, play a role in VB decolorization by P. chrysosporium.

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Authors and Affiliations

  1. National Center for Radiation Research and Technology (NCRRT), PO Box 29, Nasr City, Cairo, Egypt

    Ola M. Gomaa

  2. Department of Food Science and Human Nutrition, Michigan State University (MSU), East Lansing, MI, USA

    John E. Linz

  3. Department of Microbiology and Molecular Genetics, Michigan State University (MSU), East Lansing, MI, USA

    C. A. Reddy

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  1. Ola M. Gomaa
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  2. John E. Linz
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  3. C. A. Reddy
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Correspondence to Ola M. Gomaa.

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Gomaa, O.M., Linz, J.E. & Reddy, C.A. Decolorization of Victoria blue by the white rot fungus, Phanerochaete chrysosporium . World J Microbiol Biotechnol 24, 2349–2356 (2008). https://doi.org/10.1007/s11274-008-9750-2

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  • DOI: https://doi.org/10.1007/s11274-008-9750-2

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