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Complete genome analysis and virulence characteristics of the Louisiana West Nile virus strain LSU–AR01

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Abstract

West Nile virus (WNV) is a member of the Flaviriridae family, which can cause significant morbidity and mortality in birds, horses, and humans. The WNV–LSU–AR01 strain was isolated from a dead blue jay in Louisiana in 2001. Phylogenetic analysis using 75 full WNV genomes revealed that the LSU–AR01 strain belongs to a distinct subclade among the North American strains. The LSU–AR01 strain differed from the NY-99 prototypic strain by 26 nucleotides causing six amino acid changes. An asparagine-to-lysine change was located immediately proximal to a known CD8+T cell epitope in NS4B, while a glutamine-to-lysine change was located within a predicted CD8+T cell epitope in NS5. The LSU–AR01 strain caused pronounced neuronal necrosis, perivascular cuffing and gliosis in comparison to the NY-99-infected mice. These results suggest that the previously identified Connecticut strains may contain highly neurovirulent strains such as the LSU–AR01 that have spread in North America.

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Authors and Affiliations

  1. Division of Biotechnology and Molecular Medicine, Louisiana State University School of Veterinary Medicine (LSU SVM), Baton Rouge, LA, 70803, USA

    Arun V. Iyer, Marc J. Boudreaux, Abolghasem Baghian, Vladimir N. Chouljenko & Konstantin G. Kousoulas

  2. Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA, 70803, USA

    Arun V. Iyer, Nobuko Wakamatsu, Alma F. Roy, Abolghasem Baghian, Vladimir N. Chouljenko & Konstantin G. Kousoulas

  3. Louisiana Animal Disease Diagnostic Laboratory (LADDL), Louisiana State University School of Veterinary Medicine, Baton Rouge, LA, 70803, USA

    Alma F. Roy

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  1. Arun V. Iyer
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Iyer, A.V., Boudreaux, M.J., Wakamatsu, N. et al. Complete genome analysis and virulence characteristics of the Louisiana West Nile virus strain LSU–AR01. Virus Genes 38, 204–214 (2009). https://doi.org/10.1007/s11262-008-0321-2

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