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Interaction of diet and the masou salmon Δ5-desaturase transgene on Δ6-desaturase and stearoyl-CoA desaturase gene expression and N-3 fatty acid level in common carp (Cyprinus carpio)

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Abstract

The masou salmon Δ5-desaturase-like gene (D5D) driven by the common carp β-actin promoter was transferred into common carp (Cyprinus carpio) that were fed two diets. For P1 transgenic fish fed a commercial diet, Δ6-desaturase-like gene (D6D) and stearoyl-CoA desaturase (SCD) mRNA levels in muscle were up-regulated (P < 0.05) 12.7- and 17.9-fold, respectively, and the D6D mRNA level in the gonad of transgenic fish was up-regulated 6.9-fold (P < 0.05) compared to that of non-transgenic fish. In contrast, D6D and SCD mRNA levels in transgenic fish were dramatically down-regulated (P < 0.05), 50.2- and 16.7-fold in brain, and 5.4- and 2.4-fold in liver, respectively, in comparison with those of non-transgenic fish. When fed a specially formulated diet, D6D and SCD mRNA levels in muscle of transgenic fish were up-regulated (P < 0.05) 41.5- and 8.9-fold, respectively, and in liver 6.0- and 3.3-fold, respectively, compared to those of non-transgenic fish. In contrast, D6D and SCD mRNA levels in the gonad of transgenic fish were down-regulated (P < 0.05) 5.5- and 12.4-fold, respectively, and D6D and SCD mRNA levels in the brain were down-regulated 14.9- and 1.4-fold (P < 0.05), respectively, compared to those of non-transgenic fish. The transgenic common carp fed the commercial diet had 1.07-fold EPA, 1.12-fold DPA, 1.07-fold DHA, and 1.07-fold higher observed total omega-3 fatty acid levels than non-transgenic common carp. Although these differences were not statistically different (P > 0.05), there were significantly (P < 0.10) higher omega-3 fatty acid levels when considering the differences for all of the individual omega-3 fatty acids. The genotype × diet interactions observed indicated that the potential of desaturase transgenesis cannot be realized without using a well-designed diet with the needed amount of substrates.

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Acknowledgments

The authors thank Dr. Goro Yoshizaki of the Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan for providing the masou salmon Δ5-desaturase sequence and Dr. Pat Gibbs of Marine Biology and Fisheries, University of Miami, Miami, Florida, USA for providing the backbone plasmid, FRM2bl, for the construct in the current study.

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  1. Dayan A. Perera

    Present address: Department of Biology, West Virginia State University, Institute, WV, 25112-1000, USA

  2. Fabio Soller

    Present address: Cargill Corn Milling North America, 1705 Kellie Dr, Blair, NE, 68008, USA

Authors and Affiliations

  1. School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36839, USA

    Qi Cheng, Baofeng Su, Zhenkui Qin, Chia-Chen Weng, Yangen Zhou, Michael Fobes, Dayan A. Perera, Mei Shang, Fabio Soller, Allen Davis & Rex A. Dunham

  2. Department of Civil Engineering, Auburn University, Auburn, AL, 36839, USA

    Fang Yin

  3. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Qi Cheng & Zhiyi Shi

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  1. Qi Cheng
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Correspondence to Rex A. Dunham.

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Qi Cheng and Baofeng Su are co-first authors.

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Cheng, Q., Su, B., Qin, Z. et al. Interaction of diet and the masou salmon Δ5-desaturase transgene on Δ6-desaturase and stearoyl-CoA desaturase gene expression and N-3 fatty acid level in common carp (Cyprinus carpio). Transgenic Res 23, 729–742 (2014). https://doi.org/10.1007/s11248-014-9812-1

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