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Non-destructive estimation of root mass using electrical capacitance on ten herbaceous species

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Abstract

Introduction

The measurement of electrical capacitance across a root system has been proposed as a quick, affordable, non-invasive and non-destructive method to estimate plant root biomass. Few studies have evaluated this method and of these, most have used plants grown either in hydroponic or sand-based growth media. Our objective was to determine if measures of root electrical capacitance can provide accurate estimates the root biomass of plants grown in soil.

Aims and methods

Plants of 10 crop and forage species were grown in two sizes of pots (1.5, 4.5 L) containing a fertile potting soil. Each pot contained either one or two individuals and pots were watered to field capacity 1 h before measurements. Electrical capacitance (nanofarads) of the root system was measured and then the root system was extracted.

Results

The relationship between electrical capacitance and root dry mass was significant but too weak (r 2 = 0.30) to accurately estimate the biomass of the root systems without prior species-specific calibrations. Root interactions had significant effects on the specific capacitance response, and fine-root species seemed to be more affected.

Conclusions

Electrical capacitance in soil-based experiments did not prove as promising as previously shown with hydroponic or sand-based growing media. Targeted root traits based on biological explanations of these capacitor behaviours would need further study to increase the predictive accuracy of this method.

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References

  • Aubrecht L, Stanek Z, Koller J (2006) Electrical measurement of the absorption surfaces of tree roots by the earth impedance method: 1. Theory. Tree Physiol 26:1105–1112

    Article  PubMed  Google Scholar 

  • Cao Y, Repo T, Silvennoinen R, Lehto T, Pelkonen P (2010a) An appraisal of the electrical resistance method for assessing root surface area. J Exp Bot 61:2491–2497

    Article  PubMed  CAS  Google Scholar 

  • Cao Y, Repo T, Silvennoinen R, Lehto T, Pelkonen P (2010b) Analysis of the willow root system by electrical impedance spectroscopy. J Exp Bot 62:351–358

    Article  PubMed  Google Scholar 

  • Cermak J, Ulrich R, Stanek Z, Koller J, Aubrecht L (2006) Electrical measurement of tree root absorbing surfaces by earth impedance method: 2. Verification based on allometric relationships and root severing experiments. Tree Physiol 26:1113–1121

    Article  PubMed  Google Scholar 

  • Chloupek O (1972) The relationship between electrical capacitance and some other parameters of plant roots. Biol Plant 14:227–230

    Article  Google Scholar 

  • Chloupek O (1977) Evaluations of the size of a plant’s root system using its electrical capacitance. Plant Soil 48:525–532

    Article  Google Scholar 

  • Chloupek O, Skacel M, Ehrenbergerova J (1999) Effect of divergent selection for root size in field-grown alfalfa. Can J Plant Sci 79:93–96

    Article  Google Scholar 

  • Currie PO, Hilken TO, White RS (1987) Evaluation of a single probe capacitance meter for estimating herbage yield. J Range Manag 537–541

  • Dalton F (1995) In-situ root extent measurements by electrical capacitance methods. Plant Soil 173:157–165

    Article  CAS  Google Scholar 

  • Dvořák M, Černohorská J, Janáček K (1981) Characteristics of current passage through plant tissue. Biol Plant 23:306–310

    Article  Google Scholar 

  • Herkelrath WN, Miller EE, Gardner WR (1977) Water uptake by plants: II. The root contact model 1. Soil Sci Soc Am J 41:1039–1043

    Article  Google Scholar 

  • Kattge J, Díaz S, Lavorel S, Prentice IC, Leadley P, Bönisch G, Garnier E, Westoby M, Reich PB, Wright IJ, Cornelissen JHC, Violle C, Harrison SP, Bodegom PMv, Reichstein M, Soudzilovskaia NA, Ackerly D, Anand M, Atkin O, Bahn M, Baker TR, Baldocchi D, Bekker R, Blanco C, Blonder B, Bond W, Bradstock R, Bunker DE, Casanoves F, Cavender-Bares J, Chambers J, Chapin FS, Chave J, Coomes D, Cornwell WK, Craine JM, Duarte L, Dobrin BH, Durka W, Elser J, Enquist BJ, Esser G, Estiarte M, Fagan WF, Fang J, Fernàndez F, Fidelis A, Finegan B, Flores O, Ford H, Frank D, Freschet GT, Fyllas NM, Gallagher R, Green W, Gutierrez AG, Hickler T, Higgins S, Hodgson JG, Jalili A, Jansen S, Joly C, Kerkhoff AJ, Kirkup D, Kitajima K, Kleyer M, Klotz S, Knops JMH, Kramer K, Kühn I, Kurokawa H, Laughlin D, Lee TD, Leishman M, Lens F, Lenz T, Lewis SL, Lloyd J, Llusià J, Louault F, Ma S, Mahecha MD, Manning P, Massad T, Medlyn B, Messier J, Moles A, Müller S, Nadrowski K, Naeem S, Niinemets Ü, Nöllert S, Nüske A, Ogaya R, Oleksyn J, Onipchenko VG, Onoda Y, Ordoñez J, Overbeck G, Ozinga W, Patiño S, Paula S, Pausas JG, Peñuelas J, Phillips OL, Pillar V, Poorter H, Poorter L, Poschlod P, Proulx R, Rammig A, Reinsch S, Reu B, Sack L, Salgado B, Sardans J, Siefert A, Shiodera S, Shipley B, Sosinski E, Soussana J-F, Swaine E, Swenson N, Thompson K, Thornton P, Waldram M, Weiher E, White M, Wright SJ, Zaehle S, Zanne AE, Wirth C (2011) TRY – a global database of plant traits. Global Change Biol (in press)

  • Kendall W, Pederson G, Hill R Jr (1982) Root size estimates of red clover and alfalfa based on electrical capacitance and root diameter measurements. Grass Forage Sci 37:253–256

    Article  Google Scholar 

  • Lekas TM, MacDougal R, MacLean DA, Thompson RG (1990) Seasonal trends and effects of temperature and rainfall on stem electrical capacitance of spruce and fir trees. Can J For Res 20:970–977

    Article  Google Scholar 

  • Mcbride R, Candido M, Ferguson J (2008) Estimating root mass in maize genotypes using the electrical capacitance method. Arch Agronomy Soil Sc 54:215–226

    Article  Google Scholar 

  • Ozier-Lafontaine H, Bajazet T (2005) Analysis of root growth by impedance spectroscopy (EIS). Plant Soil 277:299–313

    Article  CAS  Google Scholar 

  • Polomski J, Kuhn N (2002) Root Research Methods. In: Waisel Y, Eshel A, Kafkafi U (eds) Plant roots: the hidden half. Marcel Drekker, New York, pp 295–321

    Google Scholar 

  • Preston GM, McBride RA, Bryan J, Candido M (2004) Estimating root mass in young hybrid poplar trees using the electrical capacitance method. Agrofor Syst 60:305–309

    Article  Google Scholar 

  • Rajkai K, Végh KR, Nacsa T (2005) Electrical capacitance of roots in relation to plant electrodes, measuring frequency and root media. Acta Agronomica Hungarica 53:197–210

    Article  Google Scholar 

  • Repo T, Laukkanen J, Silvennoinen R (2005) Measurement of the tree root growth using electrical impedance spectroscopy. Silva Fennica 39:159–166

    Google Scholar 

  • Shen J, Rengel Z, Tang C, Zhang F (2003) Role of phosphorus nutrition in development of cluster roots and release of carboxylates in soil-grown Lupinus albus. Plant Soil 248:199–206

    Article  CAS  Google Scholar 

  • Urban J, Bequet R, Mainiero R (2011) Assessing the applicability of the earth impedance method for in situ studies of tree root systems. J Exp Bot 62:1857–1869

    Article  PubMed  CAS  Google Scholar 

  • van Beem J, Smith ME, Zobel RW (1998) Estimating root mass in maize using a portable capacitance meter. Agron J 90:566–570

    Article  Google Scholar 

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Acknowledgements

This research was funded by a Natural Sciences and Engineering Research Council of Canada Strategic Grant. The authors are very grateful to Jessy Loranger and Philippe Grégoire who kindly helped in data collection and root sorting.

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Authors and Affiliations

  1. Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, J1K2R1, Canada

    Maurice Aulen & Bill Shipley

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  1. Maurice Aulen
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  2. Bill Shipley
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Correspondence to Bill Shipley.

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Responsible Editor: Alain Pierret.

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Aulen, M., Shipley, B. Non-destructive estimation of root mass using electrical capacitance on ten herbaceous species. Plant Soil 355, 41–49 (2012). https://doi.org/10.1007/s11104-011-1077-3

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