Skip to main content
SpringerLink
Log in

A Putative Plasma Membrane Cation/proton Antiporter from Soybean Confers Salt Tolerance in Arabidopsis

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Cation transport is thought to be an important process for ion homeostasis in plant cells. Here, we report that a soybean putative cation/proton antiporter GmCAX1 may be a mediator of this process. GmCAX1 is expressed in all tissues of the soybean plants but at a lower level in roots. Its expression was induced by PEG, ABA, Ca2+, Na+ and Li+ treatments. The GmCAX1-GFP fusion protein was mainly localized in plasma membrane of the transgenic Arabidopsis plant cells and onion epidermal cells. Transgenic Arabidopsis plants overexpressing GmCAX1 accumulated less Na+, K+, and Li+, and were more tolerant to elevated Li+ and Na+ levels during germination when compared with the controls. These results suggest that GmCAX1 may function as an antiporter for Na+, K+ and Li+. Modulation of this antiporter may be beneficial for regulation of ion homeostasis and thus plant salt tolerance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • M.P. Apse G.S. Aharon W.A. Snedden E. Blumwald (1999) ArticleTitleSalt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis Science 285 1256–1258 Occurrence Handle10.1126/science.285.5431.1256 Occurrence Handle10455050

    Article  PubMed  Google Scholar 

  • N. Bechtold G. Pelletier (1998) ArticleTitleIn planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration Methods Mol Biol. 82 259–266 Occurrence Handle9664431

    PubMed  Google Scholar 

  • S.Y. Chen L.H. Zhu J. Hong (1991) ArticleTitleMolecular biological identification of a rice salt tolerance line Acta. Bot. Sin. 33 569–573

    Google Scholar 

  • N.H. Cheng J.Z. Liu R.S. Nelson K.D. Hirschi (2004) ArticleTitleCharacterization of CXIP4, a novel Arabidopsis protein that activates the H+/Ca2+ antiporter, CAX1 FEBS Lett. 559 99–106 Occurrence Handle10.1016/S0014-5793(04)00036-5 Occurrence Handle14960315

    Article  PubMed  Google Scholar 

  • N.H. Cheng J.K. Pittman T. Shigaki K.D. Hirschi (2002) ArticleTitleCharacterization of CAX4, an Arabidopsis H(+)/cation antiporter Plant Physiol. 128 1245–1254 Occurrence Handle10.1104/pp.010857 Occurrence Handle11950973

    Article  PubMed  Google Scholar 

  • N.H. Cheng J.K. Pittman B.J. Barkla T. Shigaki K.D. Hirschi (2003) ArticleTitleThe Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development, and hormonal responses and reveals interplay among vacuolar transporters Plant Cell 15 347–364 Occurrence Handle10.1105/tpc.007385 Occurrence Handle12566577

    Article  PubMed  Google Scholar 

  • E.L. Connolly J.P. Fett M.L. Guerinot (2002) ArticleTitleExpression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation Plant Cell 14 1347–1357 Occurrence Handle10.1105/tpc.001263 Occurrence Handle12084831

    Article  PubMed  Google Scholar 

  • K.W. Cunningham G.R. Fink (1996) ArticleTitleCalcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+-ATPases in Saccharomyces cerevisiae Mol. Cell Biol. 16 2226–2237 Occurrence Handle8628289

    PubMed  Google Scholar 

  • E. Delhaize T. Kataoka D.M. Hebb R.G. White P.R. Ryan (2003) ArticleTitleGenes encoding proteins of the cation diffusion facilitator family that confer manganese tolerance Plant Cell 15 1131–1142 Occurrence Handle10.1105/tpc.009134 Occurrence Handle12724539

    Article  PubMed  Google Scholar 

  • W.F. Ettinger A.M. Clear K.J. Fanning M.L. Peck (1999) ArticleTitleIdentification of a Ca2+/H+ antiport in the plant chloroplast thylakoid membrane Plant Physiol. 119 1379–1385 Occurrence Handle10.1104/pp.119.4.1379 Occurrence Handle10198097

    Article  PubMed  Google Scholar 

  • T.C. Fox M.L. Guerinot (1998) ArticleTitleMolecular biology of cation transport in plants Annu. Rev. Plant Physiol. Plant Mol. Biol. 49 669–696 Occurrence Handle10.1146/annurev.arplant.49.1.669 Occurrence Handle15012250

    Article  PubMed  Google Scholar 

  • C.Y. He A.G. Tian J.S. Zhang Z.Y. Zhang J.Y. Gai S.Y. Chen (2003) ArticleTitleIsolation and characterization of a full-length resistance gene homolog from soybean Theor. Appl. Genet. 106 786–793 Occurrence Handle12647051

    PubMed  Google Scholar 

  • R. Henriques J. Jasik M. Klein E. Martinoia U. Feller J. Schell M.S. Pais C. Koncz (2002) ArticleTitleKnock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects Plant Mol. Biol. 50 587–597 Occurrence Handle10.1023/A:1019942200164 Occurrence Handle12374293

    Article  PubMed  Google Scholar 

  • K.D. Hirschi R.G. Zhen K.W. Cunningham P.A. Rea G.R. Fink (1996) ArticleTitleCAX1, an H+/Ca2+ antiporter from Arabidopsis Proc. Natl. Acad. Sci. USA 93 8782–8786 Occurrence Handle10.1073/pnas.93.16.8782 Occurrence Handle8710949

    Article  PubMed  Google Scholar 

  • K.D. Hirschi (1999) ArticleTitleExpression of Arabidopsis CAX1 in tobacco: altered calcium homeostasis and increased stress sensitivity Plant Cell 11 2113–2122 Occurrence Handle10.1105/tpc.11.11.2113 Occurrence Handle10559438

    Article  PubMed  Google Scholar 

  • K.D. Hirschi V. Korenkov N. Wilganowski G. Wagner (2000) ArticleTitleExpression of Arabidopsis CAX2 in tobacco: altered metal accumulation and increased manganese tolerance Plant Physiol. 124 125–133 Occurrence Handle10.1104/pp.124.1.125 Occurrence Handle10982428

    Article  PubMed  Google Scholar 

  • K.D. Hirschi (2001) ArticleTitleVacuolar H+/Ca2+ transport: who’s directing the traffic? Trends Plant Sci. 6 100–104 Occurrence Handle10.1016/S1360-1385(00)01863-X Occurrence Handle11239607

    Article  PubMed  Google Scholar 

  • Y. Hu Y. Wang F. Bao J.Y. Li Z. Wang Z. Peng N. Li (2001) ArticleTitleIdentification of brassinosteroid responsive genes in Arabidopsis by cDNA array Sci. in China (Ser. C) 44 637–643

    Google Scholar 

  • N. Kasai S. Muto (1990) ArticleTitleCa2+ pump and Ca2+/H+ antiporter in plasma membrane vesicles isolated by aqueous two-phase partitioning from maize leaves J. Membr. Biol. 114 133–142 Occurrence Handle10.1007/BF01869094 Occurrence Handle2160540

    Article  PubMed  Google Scholar 

  • F.J. Maathuis D. Sanders (1992) ArticleTitlePlant membrane transport Curr. Opin. Cell Biol. 4 661–669 Occurrence Handle10.1016/0955-0674(92)90087-S Occurrence Handle1384584

    Article  PubMed  Google Scholar 

  • P. Maser S. Thomine J.I. Schroeder J.M. Ward K. Hirschi H. Sze I.N. Talke A. Amtmann F.J.M. Maathuis D. Sanders (2001) ArticleTitlePhylogenetic relationships within cation transporter families of Arabidopsis Plant Physiol. 126 1646–1667 Occurrence Handle10.1104/pp.126.4.1646 Occurrence Handle11500563

    Article  PubMed  Google Scholar 

  • T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue culture Physiol. Plant. 15 473–497

    Google Scholar 

  • J.K. Pittman K.D. Hirschi (2001) ArticleTitleRegulation of CAX1, an Arabidopsis Ca2+/H+ antiporter: identification of an N-terminal autoinhibitory domain Plant Physiol. 127 1020–1029 Occurrence Handle10.1104/pp.127.3.1020 Occurrence Handle11706183

    Article  PubMed  Google Scholar 

  • J.K. Pittman C.S. Sreevidya T. Shigaki H. Ueoka-Nakanishi K.D. Hirschi (2002) ArticleTitleDistinct N-terminal regulatory domains of Ca2+/H+ antiporters Plant Physiol. 130 1054–1062 Occurrence Handle10.1104/pp.008193 Occurrence Handle12376668

    Article  PubMed  Google Scholar 

  • J. Schultz. R.R. Copley T. Doerks C.P. Ponting P. Bork (2000) ArticleTitleSMART: a Web-based tool for the study of genetically mobile domains Nucl. Acids. Res. 28 231–234 Occurrence Handle10.1093/nar/28.1.231 Occurrence Handle10592234

    Article  PubMed  Google Scholar 

  • K.S. Schumaker H. Sze (1985) ArticleTitleCa2+/H+ antiport system driven by the proton electrochemical gradient of a tonoplast H+-ATPase from oat roots Plant Physiol. 79 1111–1117

    Google Scholar 

  • O. Shaul D.W. Hilgemann J. de-Almeida-Engler M. Montagu ParticleVan D. Inze G. Galili (1999) ArticleTitleCloning and characterization of a novel Mg2+/H+ exchanger EMBO J. 18 3973–3980 Occurrence Handle10.1093/emboj/18.14.3973 Occurrence Handle10406802

    Article  PubMed  Google Scholar 

  • H.Z. Shi B.H. Lee S.J. Wu J.K. Zhu (2003) ArticleTitleOverexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana Nature biotechenlogy. 21 81–85 Occurrence Handle10.1038/nbt766

    Article  Google Scholar 

  • H. Shi M. Ishitani C. Kim J.K. Zhu (2000) ArticleTitleThe Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter Proc. Natl. Acad. Sci. USA. 97 6896–6901 Occurrence Handle10.1073/pnas.120170197 Occurrence Handle10823923

    Article  PubMed  Google Scholar 

  • T. Shigaki N.H. Cheng J.K. Pittman K.D. Hirschi (2001) ArticleTitleStructural determinants of Ca2+ transport in the Arabidopsis H+/Ca2+ antiporter CAX1 J. Biol. Chem. 276 43152–43159 Occurrence Handle10.1074/jbc.M106637200 Occurrence Handle11562366

    Article  PubMed  Google Scholar 

  • T. Shigaki K.D. Hirschi (2000) ArticleTitleCharacterization of CAX-like genes in plants: implications for functional diversity Gene 257 291–298 Occurrence Handle10.1016/S0378-1119(00)00390-5 Occurrence Handle11080595

    Article  PubMed  Google Scholar 

  • A.G. Tian J. Wang P. Cui Y.J. Han H. Xu L.J. Cong X.G. Huang X.L. Wang Y.Z. Jiao B.J. Wang Y.J. Wang J.S. Zhang S.Y. Chen (2004) ArticleTitleCharacterization of soybean genomic features by analysis of its expressed sequence tags Theor. Appl. Genet. 108 903–913 Occurrence Handle10.1007/s00122-003-1499-2 Occurrence Handle14624337

    Article  PubMed  Google Scholar 

  • H. Ueoka-Nakanishi M. Maeshima (2000) ArticleTitleQuantification of Ca2+/H+ antiporter VCAX1p in vacuolar membranes and its absence in roots of mung bean Plant Cell Physiol. 41 1067–1071 Occurrence Handle10.1093/pcp/pcd023 Occurrence Handle11100779

    Article  PubMed  Google Scholar 

  • H. Ueoka-Nakanishi Y. Nakanishim M. Maeshima (1999) ArticleTitleProperties and molecular cloning of Ca2+/H+ antiporter in the vacuolar membrane of mung bean Eur. J. Biochem. 262 417–425 Occurrence Handle10.1046/j.1432-1327.1999.00377.x Occurrence Handle10336626

    Article  PubMed  Google Scholar 

  • H. Ueoka-Nakanishi T. Tsuchiya M. Sasaki Y. Nakanishi K.W. Cunningham M. Maeshima (2000) ArticleTitleFunctional expression of mung bean Ca2+/H+ antiporter in yeast and its intracellular localization in the hypocotyl and tobacco cells Eur. J. Biochem. 267 3090–3098 Occurrence Handle10.1046/j.1432-1033.2000.01343.x Occurrence Handle10806410

    Article  PubMed  Google Scholar 

  • C. Varotto D. Maiwald P. Pesaresi P. Jahns F. Salamini D. Leister (2002) ArticleTitleThe metal ion transporter IRT1 is necessary for iron homeostasis and efficient photosynthesis in Arabidopsis thaliana Plant J. 31 589–599 Occurrence Handle10.1046/j.1365-313X.2002.01381.x Occurrence Handle12207649

    Article  PubMed  Google Scholar 

  • J.S. Zhang J. Gu F.H. Liu S.Y. Chen (1995) ArticleTitleA gene encoding a truncated large subunit of rubisco is transcribed and salt-inducible in rice Theor. Appl. Genet. 91 361–366 Occurrence Handle10.1007/BF00220900

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Guang-Zuo Luo, Hui-Wen Wang, Jian Huang, Ai-Guo Tian, Yong-Jun Wang, Jin-Song Zhang & Shou-Yi Chen

Authors
  1. Guang-Zuo Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui-Wen Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jian Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ai-Guo Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yong-Jun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jin-Song Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shou-Yi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shou-Yi Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Luo, GZ., Wang, HW., Huang, J. et al. A Putative Plasma Membrane Cation/proton Antiporter from Soybean Confers Salt Tolerance in Arabidopsis. Plant Mol Biol 59, 809–820 (2005). https://doi.org/10.1007/s11103-005-1386-0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11103-005-1386-0

Keywords

Search

Navigation