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Early Regional Response of Apoptotic Activity in Newborn Piglet Brain Following Hypoxia and Ischemia

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Abstract

Responses of selected neuroregulatory proteins that promote (Caspase 3 and Bax) or inhibit (Bcl-2, high Bcl-2/Bax ratio) apoptotic cell death were measured in the brain of piglets subjected to precisely controlled hypoxic and ischemic insults: 1 h hypoxia (decreasing FiO2 from 21 to 6%) or ischemia (ligation of carotid arteries and hemorrhage), followed by 0, 2 and 4 h recovery with 21% FiO2. Protein expression was measured in cortex, hippocampus and striatum by Western blot. There were no significant differences in expression of Caspase-3 between sham operated, hypoxic and ischemic groups. There were significant regional differences in expression of Bcl-2 and Bax in response to hypoxia and ischemia. The changes in Bcl-2/Bax ratio were similar for hypoxia and ischemia except for striatum at zero time recovery, with ischemia giving lower ratios than hypoxia. The Bcl-2/Bax ratio was also lower for the striatum than for the other regions of the brain, suggesting this region is the more susceptible to apoptotic injury.

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Acknowledgments

Supported by Grants NS-31465, HL058669, and HL081273.

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Authors and Affiliations

  1. Department of Anesthesiology and Critical Care Medicine, Children’s Hospital of Philadelphia, Philadelphia, PA, USA

    A. Pirzadeh, A. Mammen, E. Reade & W. J. Greeley

  2. Department Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, 264 Anatomy Chemistry Bldg, Philadelphia, PA, 19104, USA

    J. Kubin, H. Liu, A. Mendoza, D. F. Wilson & A. Pastuszko

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  1. A. Pirzadeh
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  2. A. Mammen
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Correspondence to A. Pastuszko.

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Pirzadeh, A., Mammen, A., Kubin, J. et al. Early Regional Response of Apoptotic Activity in Newborn Piglet Brain Following Hypoxia and Ischemia. Neurochem Res 36, 83–92 (2011). https://doi.org/10.1007/s11064-010-0267-3

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  • DOI: https://doi.org/10.1007/s11064-010-0267-3

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