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Developmental Expression of Transferrin Binding Protein in Oligodendrocyte Lineage Cells of the Embryonic Chick Spinal Cord

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Abstract

Oligodendrocytes develop from precursor cells in the neuroepithelium of the ventral ventricular zone. Oligodendrocytes in the different stages of development are characterized by expression of a number of different marker molecules such as myelin genes, growth factors, and specific antigens. We have previously identified that transferrin binding protein (TfBP), a member of heat shock protein 90 families, is a novel avian ER-associated membrane protein that is specifically localized in oligodendrocytes in adult chicken CNS. In this study we describe the developmental expression of TfBP in the embryonic chick spinal cord. A few, distinct, TfBP+ cells appeared at the lateral margin of the subventricular neuroepithelium of the spinal cord at E7. Thereafter, some TfBP+ cells, exhibited a migrative form of unipolar or bipolar shape occurred around E8 in the mantle layer, midway between the neuroepithelium and the marginal layer of the primitive spinal cord. Thereafter, the TfBP+ cells rapidly increased in number as well as their staining intensity, and overall distribution of TfBP+ cells at E15 was comparable to that of a mature spinal cord. Our observations suggest that TfBP is expressed in the subpopulation of oligodednrcyte lineage in the development and a putative role of TfBP in relation to transferrin and iron trafficking is considered.

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Acknowledgments

This study was supported by Grand R01-2003-000-10478-0 from the Korean Science and Engineering Foundation and in part by a grant(M103KV010018 04K2201 01850) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea.

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Authors and Affiliations

  1. Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul, 110-799, Korea

    Sang Wook Park, Ha Seong Lim, Eun Jung Roh, Gye Sun Jeon & Sa Sun Cho

  2. Department of Anatomy, Chungnam National University College of Medicine, 6, Munhwa-Dong, Chung-Gu, Daejeon, 301-131, Korea

    Dong Woon Kim

  3. MRC Neuroscience Institute, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul, 110-799, Korea

    Sa Sun Cho

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  1. Sang Wook Park
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  2. Ha Seong Lim
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  3. Eun Jung Roh
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  4. Dong Woon Kim
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  5. Gye Sun Jeon
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  6. Sa Sun Cho
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Correspondence to Sa Sun Cho.

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SW Park and HS Lim contributed equally to this work.

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Park, S.W., Lim, H.S., Roh, E.J. et al. Developmental Expression of Transferrin Binding Protein in Oligodendrocyte Lineage Cells of the Embryonic Chick Spinal Cord. Neurochem Res 32, 11–18 (2007). https://doi.org/10.1007/s11064-006-9216-6

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  • DOI: https://doi.org/10.1007/s11064-006-9216-6

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