Erratum to: J Neurooncol DOI 10.1007/s11060-006-9265-3
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To obtain human CD133-positive cells, we cultured freshly isolated glioma samples in vitro in stem cell media as described in the methods; under these culture conditions we observed the formation of tumor spheres in all the cultures (tumor sphere formation is a hallmark of BTSCs [15–19]). Immunofluorescent staining revealed that the tumor spheres were comprised of CD133-positive cells (Fig. 1C). After plating in commitment media to cause adherence and differentiation, tumor spheres differentiated into adherent GFAP-positive (astrocyte marker) and Tuj1-positive (neuronal marker) cells (Fig. 1E, G), which is consistent with results of other studies [15, 28] and confirms the existence of multipotent CD133-positive cells in our samples.
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The online version of the original article can be found at http://dx.doi.org/10.1007/s11060-006-9265-3
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Wu, A., Wiesner, S., Xiao, J. et al. Expression of MHC I and NK ligands on human CD133 + glioma cells: possible targets of immunotherapy. J Neurooncol 83, 133 (2007). https://doi.org/10.1007/s11060-006-9307-x
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DOI: https://doi.org/10.1007/s11060-006-9307-x