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Transfer of sclerotinia stem rot resistance from wild Brassica oleracea into B. rapa

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Abstract

Brassica crops suffer heavily from sclerotinia stem rot which is caused by the necrotrophic fungal pathogen Sclerotinia sclerotiorum. A wild B. oleracea accession, C01 (B. incana), was identified to have good resistance to S. sclerotiorum, while B. rapa was highly susceptible. In order to improve the resistance level of B. rapa, a haploid was generated from a hybridization between a B. rapa accession 6Y733 and C01, where the major resistance quantitative trait loci (QTL) have been identified from chromosome C09. Molecular-assisted selection and resistance evaluation were performed in the backcross progenies with 6Y733 as the recurrent parent. Among 17 BC1F1 plants, one individual with the highest resistance level (1.2- and 1.7-fold higher resistance than 6Y733 in leaf and stem, respectively) and carrying resistance QTL was selected to develop BC2F1. One resistant BC2F1 plant carrying 20 chromosomes and resistance loci, and having similar morphology and fertility to 6Y733, was selected from 254 BC2F1 genotypes and further self-pollinated, resulting in 145 BC2F2 individual genotypes. One individual carrying 20 chromosomes and with good fertility (98.6 %) exhibited a significant higher resistance level than the parental B. rapa line (1.4- and 1.7-fold for leaf and stem resistance, respectively). Our data suggest that it is an effective strategy to transfer sclerotinia resistance from B. oleracea into B. rapa by using a haploid strategy which could prompt the genetic exchange of the A and C genomes.

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References

  • Boland GJ, Hall R (1994) Index of host plants of Sclerotinia sclerotiorum. Can J Plant Pathol 16:93–108

    Article  Google Scholar 

  • Chalhoub B et al (2014) Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science. doi:10.1126/science.1253435

    PubMed  Google Scholar 

  • Cheung F, Trick M, Drou N, Lim YP, Park JY, Kwon SJ, Kim JA, Scott R, Pires JC, Paterson AH, Town C, Bancroft I (2009) Comparative analysis between homoeologous genome segments of Brassica napus and its progenitor species reveals extensive sequence-level divergence. Plant Cell 21:1912–1928

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Cui C, Ge X, Gautam M, Kang L, Li Z (2012) Cytoplasmic and genomic effects on meiotic pairing in Brassica hybrids and allotetraploids from pair crosses of three cultivated diploids. Genetics 191:725–738

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Diederichsen E, Frauen M, Linders EGA, Hatakeyama K, Hirai M (2009) Status and perspectives of clubroot resistance breeding in crucifer crops. J Plant Growth Regul 28:265–281

    Article  CAS  Google Scholar 

  • Inaba R, Nishio T (2002) Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1. Theor Appl Genet 105:1159–1165

    Article  PubMed  CAS  Google Scholar 

  • Leflon F, Eber J, Letanneur L, Chelysheva O, Coriton O, Huteau C, Ryder G, Barker E, Jenczewski A, Chèvre A (2006) Pairing and recombination at meiosis of Brassica rapa (AA) × Brassica napus (AACC) hybrids. Theor Appl Genet 113:1467–1480

    Article  PubMed  CAS  Google Scholar 

  • Liu S et al (2014) The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. Nat Commun. doi:10.1038/ncomms4930

    Google Scholar 

  • Mei J, Qian L, Disi JO, Yang X, Li Q, Li J, Frauen M, Cai D, Qian W (2011) Identification of resistant sources against Sclerotinia sclerotiorum in Brassica crops with emphasis on B. oleracea. Euphytica 177:393–400

    Article  Google Scholar 

  • Mei J, Wei D, Disi J, Ding Y, Liu Y, Qian W (2012) Screening resistance against Sclerotinia sclerotiorum in Brassica crops with use of detached stem assay under controlled environment. Eur J Plant Pathol 134:599–604

    Article  Google Scholar 

  • Mei J, Ding Y, Lu K, Wei D, Liu Y, Disi J, Li J, Liu L, Liu S, McKay J, Qian W (2013) Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. Theor Appl Genet 126:549–556

    Article  PubMed  CAS  Google Scholar 

  • Mei J, Liu Y, Wei D, Wittkop B, Ding Y, Li Q, Li J, Wan H, Li Z, Ge X, Frauen M, Snowdon R, Qian W, Friedt W (2015) Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step. Theor Appl Genet 128:639–644

    Article  PubMed  CAS  Google Scholar 

  • SAS Institute (1992) SAS technical report. SAS statistics software: changes and enhancements. Release 6.07

  • Schranz M, Lysak M, Mitchell-Olds T (2006) The ABC’s of comparative genomics in the Brassicaceae: building blocks of crucifer genomes. Trends Plant Sci 11:535–542

    Article  PubMed  CAS  Google Scholar 

  • Song K, Osborn T (1992) Polyphyletic origins of Brasscia napus: new evidence based on organelle and nuclear RFLP analyses. Genome 35:992–1001

    Article  Google Scholar 

  • Song K, Osborn T, Williams P (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) 1. Genome evolution of diploid and amphidiploid species. Theor Appl Genet 75:784–794

    Article  CAS  Google Scholar 

  • Song K, Osborn T, Williams P (1990) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) 3. Genome relationships in Brassica and related genera and the origin of B. oleracea and B. rapa (syn. campestris). Theor Appl Genet 779:497–506

    Google Scholar 

  • Szadkowski E, Eber F, Huteau V, Lodé M, Huneau C, Belcram H, Coriton O, Manzanares M, Delourme R, King G, Chalhoub B, Jenczewski E, Chèvre A (2010) The first meiosis of resynthesized Brassica napus, a genome blender. New Phytol 186:102–112

    Article  PubMed  CAS  Google Scholar 

  • Wen J, Tu JX, Li ZY, Fu TD, Ma CZ, Shen JX (2008) Improving ovary and embryo culture techniques for efficient resynthesis of Brassica napus from reciprocal crosses between yellow-seeded diploids B. rapa and B. oleracea. Euphytica 162:81–89

    Article  Google Scholar 

  • Yu B, Liu P, Hong D, He Q, Yang G (2010) Improvement of sclerotinia resistance of a Polima CMS restorer line of rapeseed via phenotypic selection, marker-assisted background selection and microspore culture. Plant Breed 129:39–44

    Article  Google Scholar 

  • Yu J, Zhao M, Wang X, Tong C, Huang S, Tehrim S, Liu Y, Hua W, Liu S (2013) Bolbase: a comprehensive genomics database for Brassica oleracea. BMC Genom 14:664

    Article  CAS  Google Scholar 

  • Zhao J, Meng J (2003) Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). Theor Appl Genet 106:759–764

    PubMed  Google Scholar 

  • Zhao J, Peltier AJ, Meng J, Osborn TC, Grau CR (2004) Evaluation of sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions. Plant Dis 88:1033–1039

    Article  Google Scholar 

  • Zhao JW, Udall JA, Quijada PA, Grau CR, Meng JL, Osborn TC (2006) Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. Theor Appl Genet 112:509–516

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the 973 Program (2015CB150201), Key Projects in National Science and Technology (2014BAD01B07), National Nature Science Foundation of China (31401411 and 31171585), CSTC2012ggB80008 and CSTC2014yykfA80008.

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China

    Yijuan Ding, Jiaqin Mei, Yao Liu, Lei Wang, Yuehua Li, Huafang Wan, Jiana Li & Wei Qian

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  1. Yijuan Ding
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  2. Jiaqin Mei
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  3. Yao Liu
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  4. Lei Wang
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  5. Yuehua Li
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  6. Huafang Wan
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  7. Jiana Li
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  8. Wei Qian
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Corresponding author

Correspondence to Wei Qian.

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Table S1

Information on the genome-specific markers and SSR markers flanked with QTL (Mei et al. 2013) used to track the resistance loci in the progenies (DOCX 16 kb)

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Ding, Y., Mei, J., Liu, Y. et al. Transfer of sclerotinia stem rot resistance from wild Brassica oleracea into B. rapa . Mol Breeding 35, 225 (2015). https://doi.org/10.1007/s11032-015-0392-4

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