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Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance

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Abstract

Fusarium head blight (FHB) is a destructive disease that reduces wheat grain yield and quality. To date, the quantitative trait locus on 3BS (Fhb1) from Sumai 3 has shown the largest effect on FHB resistance. Single nucleotide polymorphism (SNP) is the most common form of genetic variation and is suitable for high-throughput marker-assisted selection (MAS). We analyzed SNPs derived from 23 wheat expressed sequence tags (ESTs) that previously mapped near Fhb1 on chromosome 3BS. Using 71 Ning 7840/Clark BC7F7 recombinant inbred lines and the single-base extension method, we mapped seven SNP markers between Xgwm533 and Xgwm493, flanking markers for Fhb1. Five of the SNPs explained 45–54% of the phenotypic variation for FHB resistance. Haplotype analysis of 63 wheat accessions from eight countries based on SNPs in EST sequences, simple sequence repeats, and sequence tagged sites in the Fhb1 region identified four major groups: (1) US-Clark, (2) Asian, (3) US-Ernie, and (4) Chinese Spring. The Asian group consisted of Chinese and Japanese accessions that carry Fhb1 and could be differentiated from other groups by marker Xsnp3BS-11. All Sumai 3-related accessions formed a subgroup within the Asian group and could be sorted out by Xsnp3BS-8. The SNP markers identified in this study should be useful for MAS of Fhb1 and fine mapping to facilitate cloning of the Fhb1 resistance gene.

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Acknowledgments

This is contribution no. 11-032-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas, USA. This project is partly funded by the US Wheat and Barley Scab Initiative and the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Authors and Affiliations

  1. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    Amy N. Bernardo

  2. Plant Genetics and Biotechnology, JAAS, Nanjing, China

    Hongxiang Ma

  3. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Hongxiang Ma, Dadong Zhang & Guihua Bai

  4. Hard Winter Wheat Genetics Research Unit, USDA-ARS, Manhattan, KS, 66506, USA

    Guihua Bai

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  1. Amy N. Bernardo
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  4. Guihua Bai
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Correspondence to Guihua Bai.

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Bernardo, A.N., Ma, H., Zhang, D. et al. Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance. Mol Breeding 29, 477–488 (2012). https://doi.org/10.1007/s11032-011-9565-y

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