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Dideoxy polymorphism scanning, a gene-based method for marker development for genetic linkage mapping

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Abstract

One of the fastest growing areas of biotechnology research today is marker-assisted breeding of crops. As a prerequisite to marker assisted breeding, genetic linkage maps are currently being developed for many species. For many purposes gene-based markers are the marker type of choice. The biggest problem in genetic linkage mapping using gene-based markers is the identification of polymorphisms between the parents of the population. To improve the efficiency of marker generation, we have developed a simple, and reasonable-cost method of polymorphism detection termed dideoxy polymorphism scanning. Since most of the time required to develop a gene-based linkage map is spent in identification of useful polymorphisms, this method will significantly shorten the time required for map generation and therefore reduce the overall cost.

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Acknowledgments

This research was supported with funds provided by the Rutgers Center for Turfgrass Science, the United States Golf Association, and the United States Department of Agriculture.

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Authors and Affiliations

  1. Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ, 08901, USA

    David Rotter & Faith C. Belanger

  2. United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA

    Scott E. Warnke

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  1. David Rotter
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  2. Scott E. Warnke
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  3. Faith C. Belanger
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Correspondence to Faith C. Belanger.

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Rotter, D., Warnke, S.E. & Belanger, F.C. Dideoxy polymorphism scanning, a gene-based method for marker development for genetic linkage mapping. Mol Breeding 19, 267–274 (2007). https://doi.org/10.1007/s11032-006-9061-y

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  • DOI: https://doi.org/10.1007/s11032-006-9061-y

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