Abstract
This study examines the cytokine/chemokine profile of a 62-year-old African American male with progressive multiple sclerosis (MS). MRI images of the MS patient demonstrated generalized white matter involvement with multiple lesions in the periventricular area. A 42-plex Discovery Assay® (Eve Technologies) of the patient’s plasma and peripheral blood mononuclear cells (PBMCs) supernatant or PBMC-derived T cell supernatant samples from two separate clinic visits revealed vastly differing cytokine/chemokine levels. In addition, certain cytokine/chemokine profiles had notable differences when compared to the larger patient group or patients’ PBMCs treated with a calpain inhibitor in vitro. Interestingly, large numbers of cytokines/chemokines and growth factors in MS PBMCs are modulated by calpain inhibition, suggesting the clinical significance of these findings in designing better therapeutics against progressive MS.
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Acknowledgements
This study was made possible by grants from the Ralph H. Johnson Veterans Administration Medical Center, Charleston (1I01BX002349-01, 1 I01 BX004269-01A1) to NLB. This work was also supported by grants from the South Carolina Spinal Cord Injury Research Fund (SCIRF #2018 I-01) to AH.
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Rachel Polcyn wrote the manuscript and drew the figures. Mollie Capone performed the experiments. Denise Matzelle coordinated the patient study, collected samples, and edited the manuscript. Aljoeson Walker and Brittany Lueking evaluated the patient and provided us with the MRI images. Elizabeth Kau evaluated the patient and provided us with the patient blood samples. Azizul Haque conceived and designed the experiments and the manuscript. Naren Banik also conceived and designed the manuscript. Azizul Haque and Naren Banik also edited the manuscript. All authors reviewed and approved the final version of the manuscript.
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Polcyn, R., Capone, M., Matzelle, D. et al. Cytokine/chemokine dysregulation in progressive MS patient is apparent and can be modulated by calpain inhibition. Metab Brain Dis 35, 255–261 (2020). https://doi.org/10.1007/s11011-019-00521-1
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DOI: https://doi.org/10.1007/s11011-019-00521-1