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Magnesium–calcium signalling in rat parotid acinar cells: effects of acetylcholine

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Abstract

This study investigated the effects of extracellular Mg2+ ([Mg2+]o) on basal and acetylcholine (ACh)-evoked amylase secretion and intracellular free Ca2+ ([Ca2+]i) in rat parotid acinar cells. In a medium containing 1.1 mM [Mg2+]o, ACh evoked significant increases in amylase secretion and [Ca2+]i. Either low (0 mM) or elevated (5 and 10 mM) [Mg2+]o attenuated ACh-evoked responses. In a nominally Ca2+ free medium, elevated [Mg2+]o attenuated basal and ACh-evoked amylase secretion and [Ca2+]i. In parotid acinar cells incubated with either 0, 1.1, 5 or 10 mM [Mg2+]o, ACh evoked a gradual decrease in [Mg2+]i. These results indicate that the ACh-evoked Mg2+ efflux is an active process since Mg2+ has to move against its gradient. Either lidocaine, amiloride, N-methyl-d-glucamine, quinidine, dinitrophenol or bumetanide can elevate [Mg2+]i above basal level. In the presence of these membrane transport inhibitors, ACh still evoked a decrease in [Mg2+]i but the response was less pronounced with either [Na+]o removal or in the presence of either amiloride or quinidine. These results indicate marked interactions between Ca2+ and Mg2+ signalling in parotid acinar cells and that ACh-evoked Mg2+ transport was not dependent upon [Na+]o.

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Authors and Affiliations

  1. Department of Oral Biology, Faculty of Dental Medicine, University of Lisbon, Cidade Universitária, Lisboa, Portugal

    Antonio Mata

  2. Oral Biology Research Group, Instituto Superior de Ciências da Saúde Egas Moniz, Monte Caparica, Portugal

    Duarte Marques, João Silveira, Joana Marques & Maria F. Mesquita

  3. Department of Physiology, Cell Physiology Research Group, University of Extremadura, Cáceres, Spain

    María A. Martínez-Burgos, José A. Pariente & Gines M. Salido

  4. Department of Biological, Forensic Sciences, University of Central Lancashire, Preston, PR1 2 HE, Lancashire, England, UK

    Jaipaul Singh

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  1. Antonio Mata
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  2. Duarte Marques
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  3. María A. Martínez-Burgos
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  4. João Silveira
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  5. Joana Marques
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  7. José A. Pariente
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  8. Gines M. Salido
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  9. Jaipaul Singh
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Correspondence to Jaipaul Singh.

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Mata, A., Marques, D., Martínez-Burgos, M.A. et al. Magnesium–calcium signalling in rat parotid acinar cells: effects of acetylcholine. Mol Cell Biochem 307, 193–207 (2008). https://doi.org/10.1007/s11010-007-9599-y

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  • DOI: https://doi.org/10.1007/s11010-007-9599-y

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