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RETRACTED ARTICLE: Alternol inhibits proliferation and induces apoptosis in mouse lymphocyte leukemia (L1210) cells

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This article was retracted on 23 November 2023

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Abstract

Alternol is a novel compound purified from the fermenting products by microorganisms named as Alternaria alternata var. monosporus from the bark of Yew. In this study, we tested its effect on mouse lymphocyte leukemia L1210 cells. Alternol was found to inhibit the proliferation and induce apoptosis in L1210 cells. When the cells were treated with Alternol, chromatin condensation and phosphatidylserine externalization were observed with the down-regulation of the pro-survival gene Bcl-2 and the activation of caspase-3, caspase-9, but not caspase-8. Moreover, exposure of cells to Alternol resulted in a significant increase in reactive oxygen species (ROS) and mitochondrial transmembrane potential (ΔΨm) depolarization. Taken together, these results demonstrate that Alternol is a potent agent in inducing L1210 cells to apoptosis, which involve caspase activation and ROS generation.

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Acknowledgments

We thank Dr. Jie-Peng Chen and Mrs. Su-Lan Zhao from Strand Biotech Company Limited for Alternol and L1210 cell line. We thank Prof. Jing-Ze Wang from the Institute of Zoology, Chinese Academy of Sciences for the critical review of the manuscript.

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Authors and Affiliations

  1. Department of Pharmacology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, 100083, P.R. China

    Zhao-Zhe Liu, Xia Liu & Chang-Ling Li

  2. National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, P.R. China

    Jin Zhu, Bo Sun, Shu Liu & Shuo Geng

  3. Graduate University of The Chinese Academy of Sciences, Beijing, 100049, P.R. China

    Jin Zhu, Bo Sun, Shu Liu & Shuo Geng

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  1. Zhao-Zhe Liu
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  2. Jin Zhu
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  3. Bo Sun
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  4. Shu Liu
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  5. Shuo Geng
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  6. Xia Liu
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  7. Chang-Ling Li
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Correspondence to Chang-Ling Li.

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Liu, ZZ., Zhu, J., Sun, B. et al. RETRACTED ARTICLE: Alternol inhibits proliferation and induces apoptosis in mouse lymphocyte leukemia (L1210) cells. Mol Cell Biochem 306, 115–122 (2007). https://doi.org/10.1007/s11010-007-9560-0

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  • DOI: https://doi.org/10.1007/s11010-007-9560-0

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