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Towards the Development of Hemerythrin-Based Blood Substitutes

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Abstract

Hemerythrin is proposed as an alternative to hemoglobin-based blood substitutes. In contrast to hemoglobin, hemerythrin exhibits negligible reactivity towards oxidative and nitrosative stress agents (peroxide, nitric oxide, nitrite). Protocols for attachment of polyethylene glycol and glutaraldehyde cross-linking of Hr are described. These derivatizations appear to have favorable effects on O2 affinity and autoxidation rates for use in blood substitutes. Based on lessons learned from hemoglobin-based blood substitutes, these derivatizations should also help limit extravasation and antigenicity of a hemerythrin-based blood substitute.

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Abbreviations

Hr:

Hemerythrin

Hb:

Hemoglobin

LB/amp:

Luria–Bertani/ampicillin

OD600:

Optical density at 600 nm

SDS–PAGE:

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

PBS:

Phosphate buffer saline

PEG:

Polyethelene glycol

MS:

Methyl-PEG4-N-hydroxysuccinimide ester

TMS:

(Methyl-PEG12)3-PEG4-N-hydroxysuccinimide ester

DMSO:

Dimethylsulfoxide

GL:

Gluteraldehyde

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Acknowledgments

We thank Prof. C.C. Cooper (University of Essex, UK) for helpful discussions. Funding from the Romanian government (project PNII 565/2007) and NIH grant GM040388 (D.M.K.) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, “Babes-Bolyai” University, 11 Arany Janos str, Cluj-Napoca, 400028, Romania

    Augustin C. Mot, Alina Roman & Radu Silaghi-Dumitrescu

  2. Department of Biology, “Babes-Bolyai” University, 11 Arany Janos str, Cluj-Napoca, 400028, Romania

    Iulia Lupan

  3. Department of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX, 78249-0698, USA

    Donald M. Kurtz Jr

Authors
  1. Augustin C. Mot
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  2. Alina Roman
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  3. Iulia Lupan
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  4. Donald M. Kurtz Jr
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  5. Radu Silaghi-Dumitrescu
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Correspondence to Radu Silaghi-Dumitrescu.

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Mot, A.C., Roman, A., Lupan, I. et al. Towards the Development of Hemerythrin-Based Blood Substitutes. Protein J 29, 387–393 (2010). https://doi.org/10.1007/s10930-010-9264-2

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